A miniaturized video system for monitoring the locomotor activity of walking drosophila melanogaster in space and terrestrial settings

Omer T. Inan, Mozziyar Etemadi, Max E. Sanchez, Oana Marcu, Sharmila Bhattacharya, Gregory T A Kovacs

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A novel method is presented for monitoring movement of Drosophila melanogaster (the fruit fly) in space. Transient fly movements were captured by a 60, 2.5-cm-cubed monochrome video camera imaging flies illuminated by a uniform light source. The video signal from this camera was bandpass filtered (0.310 Hz) and amplified by an analog circuit to extract the average light changes as a function of time. The raw activity signal output of this circuit was recorded on a computer and digitally processed to extract the fly movement events from the waveform. These events corresponded to flies entering and leaving the image and were used for extracting activity parameters such as interevent duration. The efficacy of the system in quantifying locomotor activity was evaluated by varying environmental temperature and measuring the activity level of the flies. The results of this experiment matched those reported in the literature.

Original languageEnglish (US)
Article number4637870
Pages (from-to)522-524
Number of pages3
JournalIEEE Transactions on Biomedical Engineering
Volume56
Issue number2
DOIs
StatePublished - Feb 2009

Keywords

  • Activity monitor
  • Drosophila melanogaster
  • Locomotor behavior
  • Space biology

ASJC Scopus subject areas

  • Biomedical Engineering

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