A mitotic lamin B matrix induced by RanGTP required for spindle assembly

Ming Ying Tsai, Shusheng Wang, Jill M. Heidinger, Dale K. Shumaker, Stephen A. Adam, Robert D. Goldman, Yixian Zheng*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

243 Scopus citations


Mitotic spindle morphogenesis is a series of highly coordinated movements that lead to chromosome segregation and cytokinesis. We report that the intermediate filament protein lamin B, a component of the interphase nuclear lamina, functions in spindle assembly. Lamin B assembled into a matrix-like network in mitosis through a process that depended on the presence of the guanosine triphosphate-bound form of the small guanosine triphosphatase Ran. Depletion of lamin B resulted in defects in spindle assembly. Dominant negative mutant lamin B proteins that disrupt lamin B assembly in interphase nuclei also disrupted spindle assembly in mitosis. Furthermore, lamin B was essential for the formation of the mitotic matrix that tethers a number of spindle assembly factors. We propose that lamin B is a structural component of the long-sought-after spindle matrix that promotes microtubule assembly and organization in mitosis.

Original languageEnglish (US)
Pages (from-to)1887-1893
Number of pages7
Issue number5769
StatePublished - Mar 31 2006

ASJC Scopus subject areas

  • General


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