A modified assay of neutrophil function, which utilizes lysostaphin, is described. Lysostaphin is a rapid-acting muralytic enzyme specific for S. aureus. Lysostaphin does not penetrate the neutrophils and can be rapidly inactivated. Exposure of the neutrophil bacteria suspension to lysostaphin results in the elimination of a high number of extracellular bacteria in minutes. This technique differentiates defective phagocytosis from impaired intracellular killing more efficiently than other known assay methods.
|Number of pages
|The Journal of laboratory and clinical medicine
|Published - Aug 1971
ASJC Scopus subject areas
- Pathology and Forensic Medicine