A modified assay of neutrophil function: Use of lysostaphin to differentiate defective phagocytosis from impaired intracellular killing

James S. Tan, Chatrchai Watanakunakorn, John P. Phair*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

121 Scopus citations

Abstract

A modified assay of neutrophil function, which utilizes lysostaphin, is described. Lysostaphin is a rapid-acting muralytic enzyme specific for S. aureus. Lysostaphin does not penetrate the neutrophils and can be rapidly inactivated. Exposure of the neutrophil bacteria suspension to lysostaphin results in the elimination of a high number of extracellular bacteria in minutes. This technique differentiates defective phagocytosis from impaired intracellular killing more efficiently than other known assay methods.

Original languageEnglish (US)
Pages (from-to)316-322
Number of pages7
JournalThe Journal of laboratory and clinical medicine
Volume78
Issue number2
StatePublished - Aug 1971

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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