A monoclonal antibody marker for the paraboloid region of cone photoreceptors in turtle retina

Vinod P. Gaur*, William Eldred, Daniel E. Possin, P. Vijay Sarthy

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Monoclonal antibodies that specifically label one or more cell types in retina have been produced; however, only a few antibodies that, in addition, recognize distinct subcellular structures in these cells have been reported. During a search for monoclonal antibodies that bind to specific cell types in the turtle (Pseudemys scripta elegans) retina, we obtained an antibody (20-93; an IgG) that labels the inner segment of cone photoreceptors. Ultrastructural immunocytochemistry using immunogold and avidin/biotinperoxidase techniques showed that 20-93 antigen is localized to the paraboloid, a region specifically involved in glycogen metabolism in cones. In addition, a few bipolar cells were found to be labeled. The monoclonal antibody showed limited species cross-reactivity and failed to stain mouse, rat, rabbit, dog, cow, Anolis, and human retinas. Immunoblotting showed that monoclonal antibody 20-93 binds to a 40 KDa protein that is present in the retinal membrane. The antibody should be useful in immunological studies of the cone paraboloid.

Original languageEnglish (US)
Pages (from-to)497-503
Number of pages7
JournalCell and Tissue Research
Volume257
Issue number3
DOIs
StatePublished - Sep 1989

Keywords

  • Color vision
  • Monoclonal antibodies
  • Photo-receptor cells
  • Pseudemys scripta elegans (Chelonia)
  • Retina

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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