A mouse monoclonal antibody against a newly discovered basement membrane component, the epidermolysis bullosa acquisita antigen

A. S. Paller, L. L. Queen, D. T. Woodley, W. R. Gammon, E. J. O'Keefe, R. A. Briggaman

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

A mouse monoclonal antibody, H3a, directed against the newly described epidermolysis bullosa acquisita (EBA) antigen was obtained using hybridoma techniques. The distribution of the monoclonal antibody is identical to that of the polyclonal serum antibody of patients with EBA. By immunofluorescence, a linear band is seen at the dermal-epidermal junction and, by immunoelectron microscopy, immune reaction products are present in the lamina densa and sublamina densa regions. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by Western immunoblot analysis shows that the monoclonal antibody recognizes 290 and 145 kilodalton proteins present in the immunizing junctional extract, identical with the newly discovered EBA antigen. This monoclonal antibody should be useful in the further isolation and characterization of the EBA antigen.

Original languageEnglish (US)
Pages (from-to)215-217
Number of pages3
JournalJournal of Investigative Dermatology
Volume84
Issue number3
DOIs
StatePublished - 1985

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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