Abstract
The measurement of physicochemical parameters in living cells can provide information on individual cellular organelles, helping us to understand subcellular function in health and disease. While organelle-specific chemical probes have allowed qualitative evaluation of microenvironmental variations, the simultaneous quantification of mitochondrial local microviscosity (ηm) and micropolarity (ϵm), along with concurrent structural variations, has remained an unmet need. Herein, we describe a new multifunctional mitochondrial probe (MMP) for simultaneous monitoring of ηm and ϵm by fluorescence lifetime and emission intensity recordings, respectively. The MMP enables highly precise measurements of ηm and ϵm in the presence of a variety of agents perturbing cellular function, and the observed changes can also be correlated with alterations in mitochondrial network morphology and motility. This strategy represents a promising tool for the analysis of subtle changes in organellar structure.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 8891-8895 |
| Number of pages | 5 |
| Journal | Angewandte Chemie - International Edition |
| Volume | 57 |
| Issue number | 29 |
| DOIs | |
| State | Published - Jul 16 2018 |
Funding
The Canadian Institutes of Health Research (Foundation Grant to S.O.K.), the National Institutes of Health (R01GM116886) and the National Sciences and Engineering Research Council (Discovery Grant to S.O.K.) are acknowledged for support of the work done in the Kelley laboratory.
Keywords
- cellular microviscosity
- cellular polarity
- fluorescent probes
- mitochondria
ASJC Scopus subject areas
- Catalysis
- General Chemistry