A multimodal platform for nonlinear optical microscopy and microspectroscopy

Hongtao Chen; Haifeng Wang; Mikhail N. Slipchenko; Yookyung Jung; Yunzhou Shi; Jiabin Zhu; Kimberly K. Buhman; Ji Xin Cheng

doi:10.1364/OE.17.001282

A multimodal platform for nonlinear optical microscopy and microspectroscopy

Hongtao Chen, Haifeng Wang, Mikhail N. Slipchenko, Yookyung Jung, Yunzhou Shi, Jiabin Zhu, Kimberly K. Buhman, Ji Xin Cheng^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

123 Scopus citations

Abstract

Multimodal nonlinear optical microscopy is a valuable tool to study complex biological samples. We present an easy-to-operate approach to perform coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence (TPF), second harmonic generation (SHG), and third-harmonic generation (THG) imaging using a single laser source composed of an 80 MHz femtosecond (fs) laser, an optical parametric oscillator (OPO), and a PPLN crystal for frequency doubling. The platform allows vibrationally resonant CARS imaging of CH-rich myelin sheath in fresh spinal tissues and lipid bodies in live cells. Multimodal nonlinear optical imaging and microspectroscopy analysis of fresh liver tissues are demonstrated.

Original language	English (US)
Pages (from-to)	1282-1290
Number of pages	9
Journal	Optics Express
Volume	17
Issue number	3
DOIs	https://doi.org/10.1364/OE.17.001282
State	Published - Feb 2 2009
Externally published	Yes

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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abstract = "Multimodal nonlinear optical microscopy is a valuable tool to study complex biological samples. We present an easy-to-operate approach to perform coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence (TPF), second harmonic generation (SHG), and third-harmonic generation (THG) imaging using a single laser source composed of an 80 MHz femtosecond (fs) laser, an optical parametric oscillator (OPO), and a PPLN crystal for frequency doubling. The platform allows vibrationally resonant CARS imaging of CH-rich myelin sheath in fresh spinal tissues and lipid bodies in live cells. Multimodal nonlinear optical imaging and microspectroscopy analysis of fresh liver tissues are demonstrated.",

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AU - Chen, Hongtao

AU - Wang, Haifeng

AU - Slipchenko, Mikhail N.

AU - Jung, Yookyung

AU - Shi, Yunzhou

AU - Zhu, Jiabin

AU - Buhman, Kimberly K.

AU - Cheng, Ji Xin

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AB - Multimodal nonlinear optical microscopy is a valuable tool to study complex biological samples. We present an easy-to-operate approach to perform coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence (TPF), second harmonic generation (SHG), and third-harmonic generation (THG) imaging using a single laser source composed of an 80 MHz femtosecond (fs) laser, an optical parametric oscillator (OPO), and a PPLN crystal for frequency doubling. The platform allows vibrationally resonant CARS imaging of CH-rich myelin sheath in fresh spinal tissues and lipid bodies in live cells. Multimodal nonlinear optical imaging and microspectroscopy analysis of fresh liver tissues are demonstrated.

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A multimodal platform for nonlinear optical microscopy and microspectroscopy

Abstract

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