A multimodal platform for nonlinear optical microscopy and microspectroscopy

Hongtao Chen, Haifeng Wang, Mikhail N. Slipchenko, Yookyung Jung, Yunzhou Shi, Jiabin Zhu, Kimberly K. Buhman, Ji Xin Cheng*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

123 Scopus citations


Multimodal nonlinear optical microscopy is a valuable tool to study complex biological samples. We present an easy-to-operate approach to perform coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence (TPF), second harmonic generation (SHG), and third-harmonic generation (THG) imaging using a single laser source composed of an 80 MHz femtosecond (fs) laser, an optical parametric oscillator (OPO), and a PPLN crystal for frequency doubling. The platform allows vibrationally resonant CARS imaging of CH-rich myelin sheath in fresh spinal tissues and lipid bodies in live cells. Multimodal nonlinear optical imaging and microspectroscopy analysis of fresh liver tissues are demonstrated.

Original languageEnglish (US)
Pages (from-to)1282-1290
Number of pages9
JournalOptics Express
Issue number3
StatePublished - Feb 2 2009
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


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