A multimodal platform for nonlinear optical microscopy and microspectroscopy

Hongtao Chen, Haifeng Wang, Mikhail N. Slipchenko, Yookyung Jung, Yunzhou Shi, Jiabin Zhu, Kimberly K. Buhman, Ji Xin Cheng*

*Corresponding author for this work

Research output: Contribution to journalArticle

104 Scopus citations

Abstract

Multimodal nonlinear optical microscopy is a valuable tool to study complex biological samples. We present an easy-to-operate approach to perform coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence (TPF), second harmonic generation (SHG), and third-harmonic generation (THG) imaging using a single laser source composed of an 80 MHz femtosecond (fs) laser, an optical parametric oscillator (OPO), and a PPLN crystal for frequency doubling. The platform allows vibrationally resonant CARS imaging of CH-rich myelin sheath in fresh spinal tissues and lipid bodies in live cells. Multimodal nonlinear optical imaging and microspectroscopy analysis of fresh liver tissues are demonstrated.

Original languageEnglish (US)
Pages (from-to)1282-1290
Number of pages9
JournalOptics Express
Volume17
Issue number3
DOIs
StatePublished - Feb 2 2009

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Fingerprint Dive into the research topics of 'A multimodal platform for nonlinear optical microscopy and microspectroscopy'. Together they form a unique fingerprint.

  • Cite this

    Chen, H., Wang, H., Slipchenko, M. N., Jung, Y., Shi, Y., Zhu, J., Buhman, K. K., & Cheng, J. X. (2009). A multimodal platform for nonlinear optical microscopy and microspectroscopy. Optics Express, 17(3), 1282-1290. https://doi.org/10.1364/OE.17.001282