A myosin I isoform in the nucleus

L. Pestic-Dragovich*, L. Stojiljkovic, A. A. Philimonenko, G. Nowak, Y. Ke, R. E. Settlage, J. Shabanowitz, D. F. Hunt, P. Hozak, P. De Lanerolle

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

211 Scopus citations

Abstract

A nuclear isoform of myosin I β that contains a unique 16-amino acid amino-terminal extension has been identified. An affinity-purified antibody to the 16-amino acid peptide demonstrated nuclear staining. Confocal and electron microscopy revealed that nuclear myosin I β colocalized with RNA polymerase II in an α-amanitin- and actinomycin D-sensitive manner. The antibody coimmunoprecipitated RNA polymerase II and blocked in vitro RNA synthesis. This isoform of myosin I β appears to be in a complex with RNA polymerase II and may affect transcription.

Original languageEnglish (US)
Pages (from-to)337-340
Number of pages4
JournalScience
Volume290
Issue number5490
DOIs
StatePublished - Oct 13 2000

ASJC Scopus subject areas

  • General

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