A myosin I isoform in the nucleus

L. Pestic-Dragovich; L. Stojiljkovic; A. A. Philimonenko; G. Nowak; Y. Ke; R. E. Settlage; J. Shabanowitz; D. F. Hunt; P. Hozak; P. De Lanerolle

doi:10.1126/science.290.5490.337

A myosin I isoform in the nucleus

L. Pestic-Dragovich^*, L. Stojiljkovic, A. A. Philimonenko, G. Nowak, Y. Ke, R. E. Settlage, J. Shabanowitz, D. F. Hunt, P. Hozak, P. De Lanerolle

^*Corresponding author for this work

Anesthesiology

Research output: Contribution to journal › Article › peer-review

215 Scopus citations

Abstract

A nuclear isoform of myosin I β that contains a unique 16-amino acid amino-terminal extension has been identified. An affinity-purified antibody to the 16-amino acid peptide demonstrated nuclear staining. Confocal and electron microscopy revealed that nuclear myosin I β colocalized with RNA polymerase II in an α-amanitin- and actinomycin D-sensitive manner. The antibody coimmunoprecipitated RNA polymerase II and blocked in vitro RNA synthesis. This isoform of myosin I β appears to be in a complex with RNA polymerase II and may affect transcription.

Original language	English (US)
Pages (from-to)	337-340
Number of pages	4
Journal	Science
Volume	290
Issue number	5490
DOIs	https://doi.org/10.1126/science.290.5490.337
State	Published - Oct 13 2000

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10.1126/science.290.5490.337

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title = "A myosin I isoform in the nucleus",

abstract = "A nuclear isoform of myosin I β that contains a unique 16-amino acid amino-terminal extension has been identified. An affinity-purified antibody to the 16-amino acid peptide demonstrated nuclear staining. Confocal and electron microscopy revealed that nuclear myosin I β colocalized with RNA polymerase II in an α-amanitin- and actinomycin D-sensitive manner. The antibody coimmunoprecipitated RNA polymerase II and blocked in vitro RNA synthesis. This isoform of myosin I β appears to be in a complex with RNA polymerase II and may affect transcription.",

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T1 - A myosin I isoform in the nucleus

AU - Pestic-Dragovich, L.

AU - Stojiljkovic, L.

AU - Philimonenko, A. A.

AU - Nowak, G.

AU - Ke, Y.

AU - Settlage, R. E.

AU - Shabanowitz, J.

AU - Hunt, D. F.

AU - Hozak, P.

AU - De Lanerolle, P.

PY - 2000/10/13

Y1 - 2000/10/13

N2 - A nuclear isoform of myosin I β that contains a unique 16-amino acid amino-terminal extension has been identified. An affinity-purified antibody to the 16-amino acid peptide demonstrated nuclear staining. Confocal and electron microscopy revealed that nuclear myosin I β colocalized with RNA polymerase II in an α-amanitin- and actinomycin D-sensitive manner. The antibody coimmunoprecipitated RNA polymerase II and blocked in vitro RNA synthesis. This isoform of myosin I β appears to be in a complex with RNA polymerase II and may affect transcription.

AB - A nuclear isoform of myosin I β that contains a unique 16-amino acid amino-terminal extension has been identified. An affinity-purified antibody to the 16-amino acid peptide demonstrated nuclear staining. Confocal and electron microscopy revealed that nuclear myosin I β colocalized with RNA polymerase II in an α-amanitin- and actinomycin D-sensitive manner. The antibody coimmunoprecipitated RNA polymerase II and blocked in vitro RNA synthesis. This isoform of myosin I β appears to be in a complex with RNA polymerase II and may affect transcription.

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DO - 10.1126/science.290.5490.337

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AN - SCOPUS:0034644632

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VL - 290

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EP - 340

JO - Science

JF - Science

IS - 5490

ER -

A myosin I isoform in the nucleus

Abstract

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