A Neutrophil-Inspired Supramolecular Nanogel for Magnetocaloric–Enzymatic Tandem Therapy

Qi Zhang; Jiaojiao Wu; Jingjing Wang; Xia Wang; Chu Wu; Mengwei Chen; Qing Wu; Maciej S. Lesniak; Yongli Mi; Yu Cheng; Qigang Wang

doi:10.1002/anie.201915118

A Neutrophil-Inspired Supramolecular Nanogel for Magnetocaloric–Enzymatic Tandem Therapy

Qi Zhang, Jiaojiao Wu, Jingjing Wang, Xia Wang, Chu Wu, Mengwei Chen, Qing Wu, Maciej S. Lesniak, Yongli Mi, Yu Cheng^*, Qigang Wang

^*Corresponding author for this work

Neurological Surgery

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

Neutrophils can responsively release reactive oxygen species (ROS) to actively combat infections by exogenous stimulus and cascade enzyme catalyzed bio-oxidation. A supramolecular nanogel is now used as an artificial neutrophil by enzymatic interfacial self-assembly of peptides (Fmoc-Tyr(H₂PO₃)-OH) with magnetic nanoparticles (MNPs) and electrostatic loading of chloroperoxidase (CPO). The MNPs within the nanogel can elevate H₂O₂ levels in cancer cells under programmed alternating magnetic field (AMF) similar to the neutrophil activator, and the loaded CPO within protective peptides nanolayer converts the H₂O₂ into singlet oxygen (¹O₂) in a sustained manner for neutrophil-inspired tumor therapy. As a proof of concept study, both the H₂O₂ and ¹O₂ in cancer cells increase stepwise under a programmed alternating magnetic field. An active enzyme dynamic therapy by magnetically stimulated oxygen stress and sustained enzyme bio-oxidation is thus shown with studies on both cells and animals.

Original language	English (US)
Pages (from-to)	3732-3738
Number of pages	7
Journal	Angewandte Chemie - International Edition
Volume	59
Issue number	9
DOIs	https://doi.org/10.1002/anie.201915118
State	Published - Feb 24 2020

Funding

This work was supported by National Natural Science Foundation of China (No. 51873156, 81571803, 51773155, 31870997), the Thousand Talents Plan, Shanghai Municipal Education Commission Innovative Program (No.2017‐01‐07‐00‐07‐E00038), the National Key Research and Development Program (No. 2016YFA0100800), Shanghai Science and International Cooperation Program (No.16410724300), the Key Project of Shanghai science and technology commission (No. 17431906600), Major Program of Development Fund for Shanghai Zhangjiang National Innovation Demonstration Zone for Stem Cell Strategic Biobank and Stem Cell Clinical Technology Transformation Platform (No. ZJ2018‐ZD‐004) and Fundamental Research Funds for the Central Universities. This work was also supported by NIH Grant R35CA197725 to M.S.L. This work was supported by National Natural Science Foundation of China (No. 51873156, 81571803, 51773155, 31870997), the Thousand Talents Plan, Shanghai Municipal Education Commission Innovative Program (No.2017-01-07-00-07-E00038), the National Key Research and Development Program (No. 2016YFA0100800), Shanghai Science and International Cooperation Program (No.16410724300), the Key Project of Shanghai science and technology commission (No. 17431906600), Major Program of Development Fund for Shanghai Zhangjiang National Innovation Demonstration Zone for Stem Cell Strategic Biobank and Stem Cell Clinical Technology Transformation Platform (No. ZJ2018-ZD-004) and Fundamental Research Funds for the Central Universities. This work was also supported by NIH Grant R35CA197725 to M.S.L.

Keywords

bio-oxidation
cancer therapy
nanogels
reactive oxygen species
self-assembly

ASJC Scopus subject areas

General Chemistry
Catalysis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/anie.201915118

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abstract = "Neutrophils can responsively release reactive oxygen species (ROS) to actively combat infections by exogenous stimulus and cascade enzyme catalyzed bio-oxidation. A supramolecular nanogel is now used as an artificial neutrophil by enzymatic interfacial self-assembly of peptides (Fmoc-Tyr(H2PO3)-OH) with magnetic nanoparticles (MNPs) and electrostatic loading of chloroperoxidase (CPO). The MNPs within the nanogel can elevate H2O2 levels in cancer cells under programmed alternating magnetic field (AMF) similar to the neutrophil activator, and the loaded CPO within protective peptides nanolayer converts the H2O2 into singlet oxygen (1O2) in a sustained manner for neutrophil-inspired tumor therapy. As a proof of concept study, both the H2O2 and 1O2 in cancer cells increase stepwise under a programmed alternating magnetic field. An active enzyme dynamic therapy by magnetically stimulated oxygen stress and sustained enzyme bio-oxidation is thus shown with studies on both cells and animals.",

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A Neutrophil-Inspired Supramolecular Nanogel for Magnetocaloric–Enzymatic Tandem Therapy

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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