TY - JOUR
T1 - A new approach for bone marrow-derived stem cells intrapancreatic autotransplantation in diabetic rats
AU - Álvarez, Silvia Salom
AU - Jiménez, Luis Miguel
AU - Murillo, Ana Zugasti
AU - Gómez, Ignacio García
AU - Ligero, José Manuel
AU - Gómez-Pineda, Alfonso
AU - Rollán-Landeras, Eduardo
AU - Cuevas, Pedro
AU - Jara-Albarrán, Antonino
PY - 2006
Y1 - 2006
N2 - Purpose: A new technique for stem cells intrapancreatic autotransplantation in rats. Basic Procedures: Section of a femoral diaphysis and aspirations of the bone marrow in both femoral segments were performed. A Kirschner needle was placed into the femur. Cells were isolated in Ficoll gradients and preserved at 4-6°C. The second day, cells were injected, via aortic celiac trunk, into the pancreas of the same rat. Results: Femoral surgery were well tolerated. Intrapancreatic homing of the injected cells was suggested with methylene blue injection that stained the pancreas, and proved by labeled cells in pancreas sections. Cell counts after Ficoll isolation were 1 × 106 ± 2 × 105 ES. Conclusions: A technique is described for stem cell autotransplantation in rats. First we obtain autologous bone marrow-derived stem cells. Second, we inject the cells in the pancreas of the donor rat. This approach can be applied to experimental diabetes and other pancreatic processes.
AB - Purpose: A new technique for stem cells intrapancreatic autotransplantation in rats. Basic Procedures: Section of a femoral diaphysis and aspirations of the bone marrow in both femoral segments were performed. A Kirschner needle was placed into the femur. Cells were isolated in Ficoll gradients and preserved at 4-6°C. The second day, cells were injected, via aortic celiac trunk, into the pancreas of the same rat. Results: Femoral surgery were well tolerated. Intrapancreatic homing of the injected cells was suggested with methylene blue injection that stained the pancreas, and proved by labeled cells in pancreas sections. Cell counts after Ficoll isolation were 1 × 106 ± 2 × 105 ES. Conclusions: A technique is described for stem cell autotransplantation in rats. First we obtain autologous bone marrow-derived stem cells. Second, we inject the cells in the pancreas of the donor rat. This approach can be applied to experimental diabetes and other pancreatic processes.
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U2 - 10.1002/micr.20283
DO - 10.1002/micr.20283
M3 - Article
C2 - 17006956
AN - SCOPUS:33750835147
SN - 0738-1085
VL - 26
SP - 539
EP - 542
JO - Microsurgery
JF - Microsurgery
IS - 7
ER -
