A new approach for bone marrow-derived stem cells intrapancreatic autotransplantation in diabetic rats

Silvia Salom Álvarez, Luis Miguel Jiménez, Ana Zugasti Murillo, Ignacio García Gómez, José Manuel Ligero, Alfonso Gómez-Pineda, Eduardo Rollán-Landeras, Pedro Cuevas, Antonino Jara-Albarrán*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Purpose: A new technique for stem cells intrapancreatic autotransplantation in rats. Basic Procedures: Section of a femoral diaphysis and aspirations of the bone marrow in both femoral segments were performed. A Kirschner needle was placed into the femur. Cells were isolated in Ficoll gradients and preserved at 4-6°C. The second day, cells were injected, via aortic celiac trunk, into the pancreas of the same rat. Results: Femoral surgery were well tolerated. Intrapancreatic homing of the injected cells was suggested with methylene blue injection that stained the pancreas, and proved by labeled cells in pancreas sections. Cell counts after Ficoll isolation were 1 × 106 ± 2 × 105 ES. Conclusions: A technique is described for stem cell autotransplantation in rats. First we obtain autologous bone marrow-derived stem cells. Second, we inject the cells in the pancreas of the donor rat. This approach can be applied to experimental diabetes and other pancreatic processes.

Original languageEnglish (US)
Pages (from-to)539-542
Number of pages4
JournalMicrosurgery
Volume26
Issue number7
DOIs
StatePublished - 2006
Externally publishedYes

ASJC Scopus subject areas

  • Surgery

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