A method has been developed for growing cultured cells in 200-μl capillary pipets in order to measure the uptake and release of materials by the cells. Feeding is accomplished by means of continuous superfusion of culture medium over the cells. The technique virtually eliminates problems caused by cultured cells detaching from the substratum during experiments and has been used to study the depolarization-dependent processes of [3H]-acetylcholine release and 22Na+ uptake by NG108-15 neuroblastoma × glioma hybrid cells.
|Original language||English (US)|
|Number of pages||7|
|State||Published - Jan 15 1980|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology