A new capillary tube system for measuring the uptake and release of materials from cultured cells

Richard McGee*, Carolyn Smith, Clifford Christian, Marina Mata, Phillip Nelson, Marshall Nirenberg

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

A method has been developed for growing cultured cells in 200-μl capillary pipets in order to measure the uptake and release of materials by the cells. Feeding is accomplished by means of continuous superfusion of culture medium over the cells. The technique virtually eliminates problems caused by cultured cells detaching from the substratum during experiments and has been used to study the depolarization-dependent processes of [3H]-acetylcholine release and 22Na+ uptake by NG108-15 neuroblastoma × glioma hybrid cells.

Original languageEnglish (US)
Pages (from-to)320-326
Number of pages7
JournalAnalytical Biochemistry
Volume101
Issue number2
DOIs
StatePublished - Jan 15 1980

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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