A new capillary tube system for measuring the uptake and release of materials from cultured cells

Richard McGee; Carolyn Smith; Clifford Christian; Marina Mata; Phillip Nelson; Marshall Nirenberg

doi:10.1016/0003-2697(80)90194-3

A new capillary tube system for measuring the uptake and release of materials from cultured cells

Richard McGee^*, Carolyn Smith, Clifford Christian, Marina Mata, Phillip Nelson, Marshall Nirenberg

^*Corresponding author for this work

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Abstract

A method has been developed for growing cultured cells in 200-μl capillary pipets in order to measure the uptake and release of materials by the cells. Feeding is accomplished by means of continuous superfusion of culture medium over the cells. The technique virtually eliminates problems caused by cultured cells detaching from the substratum during experiments and has been used to study the depolarization-dependent processes of [³H]-acetylcholine release and ²²Na⁺ uptake by NG108-15 neuroblastoma × glioma hybrid cells.

Original language	English (US)
Pages (from-to)	320-326
Number of pages	7
Journal	Analytical Biochemistry
Volume	101
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(80)90194-3
State	Published - Jan 15 1980

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "A method has been developed for growing cultured cells in 200-μl capillary pipets in order to measure the uptake and release of materials by the cells. Feeding is accomplished by means of continuous superfusion of culture medium over the cells. The technique virtually eliminates problems caused by cultured cells detaching from the substratum during experiments and has been used to study the depolarization-dependent processes of [3H]-acetylcholine release and 22Na+ uptake by NG108-15 neuroblastoma × glioma hybrid cells.",

author = "Richard McGee and Carolyn Smith and Clifford Christian and Marina Mata and Phillip Nelson and Marshall Nirenberg",

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AU - McGee, Richard

AU - Smith, Carolyn

AU - Christian, Clifford

AU - Mata, Marina

AU - Nelson, Phillip

AU - Nirenberg, Marshall

PY - 1980/1/15

Y1 - 1980/1/15

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AB - A method has been developed for growing cultured cells in 200-μl capillary pipets in order to measure the uptake and release of materials by the cells. Feeding is accomplished by means of continuous superfusion of culture medium over the cells. The technique virtually eliminates problems caused by cultured cells detaching from the substratum during experiments and has been used to study the depolarization-dependent processes of [3H]-acetylcholine release and 22Na+ uptake by NG108-15 neuroblastoma × glioma hybrid cells.

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ER -

A new capillary tube system for measuring the uptake and release of materials from cultured cells

Abstract

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