A New Method For Scanning Electron Microscopic Visualization Of Dermal Elastic Fibres

Takuo Tsuji*, Robert M. Lavker, Albert M. Kligman

*Corresponding author for this work

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Autoclaving sections of dermis for 8 h followed by fixation, dehydration and xylol‐air drying yields a pure preparation of elastic fibres for scanning electron microscopy which retains the native architecture of this component. Elastic fibres were intertwined in a complex fashion with numerous branches. Fibres were predominantly cylindrical in the upper dermis and became larger and more elliptical in the deeper dermis. This method produces a means to study the organization of elastic fibres in a variety of disorders in which dermal changes are prominent. 1979 Blackwell Science Ltd

Original languageEnglish (US)
Pages (from-to)165-173
Number of pages9
JournalJournal of Microscopy
Volume115
Issue number2
DOIs
StatePublished - Jan 1 1979

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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