A novel adenoviral vector labeled with superparamagnetic iron oxide nanoparticles for real-time tracking of viral delivery

Jonathan Yun, Adam M. Sonabend, Ilya V. Ulasov, Dong Hyun Kim, Elena A. Rozhkova, Valentyn Novosad, Stephen Dashnaw, Truman Brown, Peter Canoll, Jeffrey N. Bruce, MacIej S. Lesniak*

*Corresponding author for this work

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

In vivo tracking of gene therapy vectors challenges the investigation and improvement of biodistribution of these agents in the brain, a key feature for their targeting of infiltrative malignant gliomas. The glioma-targeting Ad5/3-cRGD gene therapy vector was covalently bound to super-paramagnetic iron oxide (Fe3O4) nanoparticles (SPION) to monitor its distribution by MRI. Transduction of labeled and unlabeled vectors was assessed on the U87 glioma cell line and normal human astrocytes (NHA), and was higher in U87 compared to NHA, but was similar between labeled and unlabeled virus. An in vivo study was performed by intracranial subcortical injection of labeled-Ad5/3-cRGD particles into a pig brain. The labeled vector appeared in vivo as a T2-weighted hyperintensity and a T2-gradient echo signal at the injection site, persisting up to 72 hours post-injection. We describe a glioma-targeting vector that is labeled with SPION, thereby allowing for MRI detection with no change in transduction capability.

Original languageEnglish (US)
Pages (from-to)875-880
Number of pages6
JournalJournal of Clinical Neuroscience
Volume19
Issue number6
DOIs
StatePublished - Jun 1 2012

Keywords

  • Adenovirus
  • Gene therapy
  • Nanoparticle

ASJC Scopus subject areas

  • Surgery
  • Neurology
  • Clinical Neurology
  • Physiology (medical)

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