@article{b52c79a37bed4d37b48aed68ba43e88c,
title = "A novel clathrin adaptor complex mediates basolateral targeting in polarized epithelial cells",
abstract = "Although polarized epithelial cells are well known to maintain distinct apical and basolateral plasma membrane domains, the mechanisms responsible for targeting membrane proteins to the apical or basolateral surfaces have remained elusive. We have identified a novel form of the AP-1 clathrin adaptor complex that contains as one of its subunits μ1B, an epithelial cell-specific homolog of the ubiquitously expressed μ1A. LLC-PK1 kidney epithelial cells do not express μ1B and missort many basolateral proteins to the apical surface. Stable expression of μ1B selectively restored basolateral targeting, improved the overall organization of LLC-PK1 monolayers, and had no effect on apical targeting. We conclude that basolateral sorting is mediated by an epithelial cell-specific version of the AP-1 complex containing μ1B.",
author = "Heike F{\"o}lsch and Hiroshi Ohno and Bonifacino, {Juan S.} and Ira Mellman",
note = "Funding Information: We would like to thank Michael Hull for his expertise and assistance in preparing recombinant adenoviruses, Moses Chao (Skirball Institute) and James Wilson (University of Pennsylvania) for providing the p75 NTR and LDL receptor adenovirus stocks, respectively, Michael Caplan (Yale University) for providing the LLC-PK1 cells and antibodies to Na,K-ATPase, and Linton Traub (Washington University) for anti-μ1A antibodies. We are indebted to Michael Caplan and Graham Warren for helpful discussions, and to the entire Mellman/Warren laboratory for their support. This work was funded by a postdoctoral fellowship from the Deutsche Forschungsgemeinschaft (to H. F.) and research grants from the National Institutes of Health (GM-29765) to I. M., and by Grant-in-Aids for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan (to H. O.). I. M. is an Affiliate Member of the Ludwig Institute for Cancer Research.",
year = "1999",
doi = "10.1016/S0092-8674(00)81650-5",
language = "English (US)",
volume = "99",
pages = "189--198",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "2",
}