A novel mouse model overexpressing Nocturnin results in decreased fat mass in male mice

Phuong T. Le, Sheila A. Bornstein, Katherine J. Motyl, Li Tian, Jeremy J. Stubblefield, Hee Kyung Hong, Joseph S. Takahashi, Carla B. Green, Clifford J. Rosen, Anyonya R. Guntur*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Nocturnin (NOCT) belongs to the Mg2+ dependent Exonucleases, Endonucleases, Phosphatase (EEP) family of enzymes that exhibit various functions in vitro and in vivo. NOCT is known to function as a deadenylase, cleaving poly-A tails from mRNA (messenger RNA) transcripts. Previously, we reported a role for NOCT in regulating bone marrow stromal cell differentiation through its interactions with PPARγ. In this study, we characterized the skeletal and adipose tissue phenotype when we globally overexpressed Noct in vivo. After 12 weeks of Noct overexpression, transgenic male mice had lower fat mass compared to controls, with no significant differences in the skeleton. Based on the presence of a mitochondrial target sequence in NOCT, we determined that mouse NOCT protein localizes to the mitochondria; subsequently, we found that NOCT overexpression led to a significant increase in the preadipocytes ability to utilize oxidative phosphorylation for ATP (adenosine triphosphate) generation. In summary, the effects of NOCT on adipocytes are likely through its novel role as a mediator of mitochondrial function.

Original languageEnglish (US)
Pages (from-to)20228-20239
Number of pages12
JournalJournal of Cellular Physiology
Issue number11
StatePublished - Nov 2019


  • Nocturnin
  • adipogenesis and osteogenesis
  • bone mass

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology


Dive into the research topics of 'A novel mouse model overexpressing Nocturnin results in decreased fat mass in male mice'. Together they form a unique fingerprint.

Cite this