A novel mutation in myelin-deficient mice results in unstable myelin basic protein gene transcripts

Brian Popko; Carmie Puckett; Leroy Hood

doi:10.1016/0896-6273(88)90142-0

A novel mutation in myelin-deficient mice results in unstable myelin basic protein gene transcripts

Brian Popko^*, Carmie Puckett, Leroy Hood

^*Corresponding author for this work

Neurology

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Mice homozygous for the myelin-deficient (mld) mutation have an unusual phenotype in which the gene encoding myelin basic protein (MBP) is expressed at low levels and on an abnormal developmental schedule. In this report we describe the organization of the mld MBP locus, which results in this alteration of MBP expression. The mld MBP locus consists of two tandem MBP genes, with the upstream gene containing an inversion of its 3′ region. We also demonstrate that although there are low steady-state levels of MBP RNA in mld mice, the mid MBP locus is transcribed at a rate comparable to that of the wild-type MBP gene, indicating that the MBP transcripts are abnormally unstable.

Original language	English (US)
Pages (from-to)	221-225
Number of pages	5
Journal	Neuron
Volume	1
Issue number	3
DOIs	https://doi.org/10.1016/0896-6273(88)90142-0
State	Published - May 1988

Funding

Ws thank Michael Greenberg and David Anderson for advice with the nuclear run-off experiments, numerous members of the Hood lab for helpful discussion, Rick Barth, Pat Concannon, Ulf Lan-degren, and Carol Readhead for crirically reviewing the manuscript, and Cathy Elkins for preparation the manuscript. This work was supported by the NIH.

ASJC Scopus subject areas

General Neuroscience

Access to Document

10.1016/0896-6273(88)90142-0

Cite this

@article{619c39accdfc40b8b5615dd7e9a9ef3b,

title = "A novel mutation in myelin-deficient mice results in unstable myelin basic protein gene transcripts",

abstract = "Mice homozygous for the myelin-deficient (mld) mutation have an unusual phenotype in which the gene encoding myelin basic protein (MBP) is expressed at low levels and on an abnormal developmental schedule. In this report we describe the organization of the mld MBP locus, which results in this alteration of MBP expression. The mld MBP locus consists of two tandem MBP genes, with the upstream gene containing an inversion of its 3′ region. We also demonstrate that although there are low steady-state levels of MBP RNA in mld mice, the mid MBP locus is transcribed at a rate comparable to that of the wild-type MBP gene, indicating that the MBP transcripts are abnormally unstable.",

author = "Brian Popko and Carmie Puckett and Leroy Hood",

note = "Funding Information: Ws thank Michael Greenberg and David Anderson for advice with the nuclear run-off experiments, numerous members of the Hood lab for helpful discussion, Rick Barth, Pat Concannon, Ulf Lan-degren, and Carol Readhead for crirically reviewing the manuscript, and Cathy Elkins for preparation the manuscript. This work was supported by the NIH.",

year = "1988",

month = may,

doi = "10.1016/0896-6273(88)90142-0",

language = "English (US)",

volume = "1",

pages = "221--225",

journal = "Neuron",

issn = "0896-6273",

publisher = "Cell Press",

number = "3",

}

TY - JOUR

T1 - A novel mutation in myelin-deficient mice results in unstable myelin basic protein gene transcripts

AU - Popko, Brian

AU - Puckett, Carmie

AU - Hood, Leroy

N1 - Funding Information: Ws thank Michael Greenberg and David Anderson for advice with the nuclear run-off experiments, numerous members of the Hood lab for helpful discussion, Rick Barth, Pat Concannon, Ulf Lan-degren, and Carol Readhead for crirically reviewing the manuscript, and Cathy Elkins for preparation the manuscript. This work was supported by the NIH.

PY - 1988/5

Y1 - 1988/5

N2 - Mice homozygous for the myelin-deficient (mld) mutation have an unusual phenotype in which the gene encoding myelin basic protein (MBP) is expressed at low levels and on an abnormal developmental schedule. In this report we describe the organization of the mld MBP locus, which results in this alteration of MBP expression. The mld MBP locus consists of two tandem MBP genes, with the upstream gene containing an inversion of its 3′ region. We also demonstrate that although there are low steady-state levels of MBP RNA in mld mice, the mid MBP locus is transcribed at a rate comparable to that of the wild-type MBP gene, indicating that the MBP transcripts are abnormally unstable.

AB - Mice homozygous for the myelin-deficient (mld) mutation have an unusual phenotype in which the gene encoding myelin basic protein (MBP) is expressed at low levels and on an abnormal developmental schedule. In this report we describe the organization of the mld MBP locus, which results in this alteration of MBP expression. The mld MBP locus consists of two tandem MBP genes, with the upstream gene containing an inversion of its 3′ region. We also demonstrate that although there are low steady-state levels of MBP RNA in mld mice, the mid MBP locus is transcribed at a rate comparable to that of the wild-type MBP gene, indicating that the MBP transcripts are abnormally unstable.

UR - http://www.scopus.com/inward/record.url?scp=0024002710&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024002710&partnerID=8YFLogxK

U2 - 10.1016/0896-6273(88)90142-0

DO - 10.1016/0896-6273(88)90142-0

M3 - Article

C2 - 2483096

AN - SCOPUS:0024002710

SN - 0896-6273

VL - 1

SP - 221

EP - 225

JO - Neuron

JF - Neuron

IS - 3

ER -

A novel mutation in myelin-deficient mice results in unstable myelin basic protein gene transcripts

Abstract

Funding

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this