A novel nuclear protein, 5qNCA (LOC51780) is a candidate for the myeloid leukemia tumor suppressor gene on chromosome 5 band q31

Zhenbo Hu, Ignatius Gomes, Stephen K. Horrigan, Jelena Kravarusic, Brenton Mar, Zarema Arbieva, Brent Chyna, Noreen Fulton, Seby Edassery, Azra Raza, Carol A. Westbrook*

*Corresponding author for this work

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

Interstitial deletion or loss of chromosome 5, del(5q) or -5, is a frequent finding in myeloid leukemias and myelodysplasias, suggesting the presence of a tumor suppressor gene within the deleted region. In our search for this gene, we identified a candidate, 5qNCA (LOC51780), which lies within a consistently-deleted segment of 5q31. 5qNCA expresses a 7.2-kb transcript with a 5286-bp open reading frame which is present at high levels in heart, skeletal muscle, kidney, placenta, and liver as well as CD34 + cells and AML cell lines. 5qNCA encodes a 191-kD nuclear protein which contains a highly-conserved C-terminus containing a zinc finger with the unique spacing Cys-X2-Cys-X7-His-X2-Cys-X2-Cys-X4-Cys-X2-Cys and a jmjC domain, which is often found in proteins that regulate chromatin remodeling. Expression of 5qNCA in a del(5q) cell line results in suppression of clonogenic growth. Preliminary sequence results in AML and MDS samples and cell lines has revealed a possible mutation in the KG-1 cell line resulting in a THR to ALA substitution that has not been found in over 100 normal alleles to date. We propose 5qNCA is a good candidate for the del(5q) tumor suppressor gene based on its predicted function and growth suppressive activities, and suggest that further mutational and functional study of this interesting gene is warranted.

Original languageEnglish (US)
Pages (from-to)6946-6954
Number of pages9
JournalOncogene
Volume20
Issue number47
DOIs
StatePublished - Oct 18 2001

Keywords

  • 5qNCA
  • Leukemia
  • Nuclear protein
  • Tumor suppressor
  • Zinc finger
  • jmjC domain

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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    Hu, Z., Gomes, I., Horrigan, S. K., Kravarusic, J., Mar, B., Arbieva, Z., Chyna, B., Fulton, N., Edassery, S., Raza, A., & Westbrook, C. A. (2001). A novel nuclear protein, 5qNCA (LOC51780) is a candidate for the myeloid leukemia tumor suppressor gene on chromosome 5 band q31. Oncogene, 20(47), 6946-6954. https://doi.org/10.1038/sj.onc.1204850