A novel podoplanin-GFPCre mouse strain for gene deletion in lymphatic endothelial cells

Hyea Jin Gil, Wanshu Ma, Guillermo Oliver*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The lymphatic vascular system is a one-direction network of thin-walled capillaries and larger vessels covered by a continuous layer of endothelial cells responsible for maintaining fluid homeostasis. Some of the main functions of the lymphatic vasculature are to drain fluid from the extracellular spaces and return it back to the blood circulation, lipid absorption from the intestinal tract, and transport of immune cells to lymphoid organs. A number of genes controlling the development of the mammalian lymphatic vasculature have been identified in the last few years, and their functional roles started to be characterized using gene inactivation approaches in mice. Unfortunately, only few mouse Cre strains relatively specific for lymphatic endothelial cells (LECs) are currently available. In this article, we report the generation of a novel Podoplanin (Pdpn) GFPCre transgenic mouse strain using its 5’ regulatory region. Pdpn encodes a transmembrane mucin-type O-glycoprotein that is expressed on the surface of embryonic and postnatal LECs, in addition to few other cell types. Our detailed characterization of this novel strain indicates that it will be a valuable additional genetic tool for the analysis of gene function in LECs.

Original languageEnglish (US)
Article numbere23102
JournalGenesis
Volume56
Issue number4
DOIs
StatePublished - Apr 2018

Keywords

  • Cre recombinase
  • Podoplanin
  • lymphatic endothelial cells
  • mouse

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

Fingerprint

Dive into the research topics of 'A novel podoplanin-GFPCre mouse strain for gene deletion in lymphatic endothelial cells'. Together they form a unique fingerprint.

Cite this