Abstract
Stimulation of dopaminergic type 1 (D1) receptors increases lung edema clearance by regulating Na,K-ATPase function in the alveolar epithelium. We studied the role of serine/threonine protein phosphatases in the Na,K-ATPase regulation by D1 agonists in A549 cells. We found that low doses of the type 1/2A protein phosphatase inhibitor okadaic acid as well as SV40 small t antigen transiently transfected into A549 cells prevented the D1 agonist- induced increase in Na,K-ATPase activity and translocation from intracellular pools to the plasma membrane. This was associated with a rapid and transient increase in protein phosphatase 2A activity. We conclude that D1 stimulation regulates Na,K-ATPase activity by promoting recruitment of Na,K-ATPases from intracellular pools into the basolateral membranes of A549 cells via a type 2A protein phosphatase. (C) 2000 Federation of European Biochemical Societies.
Original language | English (US) |
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Pages (from-to) | 217-220 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 481 |
Issue number | 3 |
DOIs | |
State | Published - Sep 22 2000 |
Keywords
- A549 cell
- D agonist
- Na,K-ATPase
- Protein phosphatase
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology