A novel role for protein phosphatase 2A in the dopaminergic regulation of Na,K-ATPase

Emilia Lecuona, Alphonse Garcia, Jacob I. Sznajder*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Stimulation of dopaminergic type 1 (D1) receptors increases lung edema clearance by regulating Na,K-ATPase function in the alveolar epithelium. We studied the role of serine/threonine protein phosphatases in the Na,K-ATPase regulation by D1 agonists in A549 cells. We found that low doses of the type 1/2A protein phosphatase inhibitor okadaic acid as well as SV40 small t antigen transiently transfected into A549 cells prevented the D1 agonist- induced increase in Na,K-ATPase activity and translocation from intracellular pools to the plasma membrane. This was associated with a rapid and transient increase in protein phosphatase 2A activity. We conclude that D1 stimulation regulates Na,K-ATPase activity by promoting recruitment of Na,K-ATPases from intracellular pools into the basolateral membranes of A549 cells via a type 2A protein phosphatase. (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)217-220
Number of pages4
JournalFEBS Letters
Volume481
Issue number3
DOIs
StatePublished - Sep 22 2000

Keywords

  • A549 cell
  • D agonist
  • Na,K-ATPase
  • Protein phosphatase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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