TY - JOUR
T1 - A novel role of CD4 Th17 cells in mediating cardiac allograft rejection and vasculopathy
AU - Yuan, Xueli
AU - Paez-Cortez, Jesus
AU - Schmitt-Knosalla, Isabela
AU - D'Addio, Francesca
AU - Mfarrej, Bechara
AU - Donnarumma, Michela
AU - Habicht, Antje
AU - Clarkson, Michael R.
AU - Iacomini, John
AU - Glimcher, Laurie H.
AU - Sayegh, Mohamed H.
AU - Javeed Ansari, M.
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2008/12/22
Y1 - 2008/12/22
N2 - T-bet plays a crucial role in Th1 development. We investigated the role of T-bet in the development of allograft rejection in an established MHC class II-mismatched (bm12 into B6) model of chronic allograft vasculopathy (CAV). Intriguingly, and in contrast to IFN-γ -/- mice that are protected from CAV, T-bet -/- recipients develop markedly accelerated allograft rejection accompanied by early severe vascular inflammation and vasculopathy, and infiltration by predominantly IL-17-producing CD4 T cells. Concurrently, T-bet -/- mice exhibit a T helper type 1 (Thl)-deficient environment characterized by profound IFN-7 deficiency, a Th2 switch characterized by increased production of interleukin (IL) 4, IL-5, IL-10, and IL-13 cytokines, as well as increased production of the proinflammatory cytokines IL-6, IL-12p40, and IL-17. Neutralization of IL-17 inhibits accelerated allograft rejection and vasculopathy in T-bet -/- mice. Interestingly, CD4 but not CD8 T cell deficiency in T-bet -/- mice affords dramatic protection from vasculopathy and facilitates long-term graft acceptance. This is the first study establishing that in the absence of Th1-mediated alloimmune responses, CD4 Th17 cells mediate an aggressive proinflamma-tory response culminating in severe accelerated allograft rejection and vasculopathy. These results have important implications for the development of novel therapies to target this intractable problem in clinical solid organ transplantation.
AB - T-bet plays a crucial role in Th1 development. We investigated the role of T-bet in the development of allograft rejection in an established MHC class II-mismatched (bm12 into B6) model of chronic allograft vasculopathy (CAV). Intriguingly, and in contrast to IFN-γ -/- mice that are protected from CAV, T-bet -/- recipients develop markedly accelerated allograft rejection accompanied by early severe vascular inflammation and vasculopathy, and infiltration by predominantly IL-17-producing CD4 T cells. Concurrently, T-bet -/- mice exhibit a T helper type 1 (Thl)-deficient environment characterized by profound IFN-7 deficiency, a Th2 switch characterized by increased production of interleukin (IL) 4, IL-5, IL-10, and IL-13 cytokines, as well as increased production of the proinflammatory cytokines IL-6, IL-12p40, and IL-17. Neutralization of IL-17 inhibits accelerated allograft rejection and vasculopathy in T-bet -/- mice. Interestingly, CD4 but not CD8 T cell deficiency in T-bet -/- mice affords dramatic protection from vasculopathy and facilitates long-term graft acceptance. This is the first study establishing that in the absence of Th1-mediated alloimmune responses, CD4 Th17 cells mediate an aggressive proinflamma-tory response culminating in severe accelerated allograft rejection and vasculopathy. These results have important implications for the development of novel therapies to target this intractable problem in clinical solid organ transplantation.
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U2 - 10.1084/jem.20081937
DO - 10.1084/jem.20081937
M3 - Article
C2 - 19047438
AN - SCOPUS:59649110032
VL - 205
SP - 3133
EP - 3144
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
SN - 0022-1007
IS - 13
ER -