A novel subgenomic murine leukemia virus RNA transcript results from alternative splicing

Jérôme Déjardin; Guillaume Bompard-Maréchal; Muriel Audit; Thomas J. Hope; Marc Sitbon; Marylène Mougel

doi:10.1128/JVI.74.8.3709-3714.2000

A novel subgenomic murine leukemia virus RNA transcript results from alternative splicing

Jérôme Déjardin, Guillaume Bompard-Maréchal, Muriel Audit, Thomas J. Hope, Marc Sitbon, Marylène Mougel^*

^*Corresponding author for this work

Cell & Developmental Biology

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Here we show the existence of a novel subgenomic 4.4-kb RNA in cells infected with the prototypic replication-competent Friend or Moloney murine leukemia viruses (MuLV). This RNA derives by splicing from an alternative donor site (SD') within the capsid-coding region to the canonical envelope splice acceptor site. The position and the sequence of SD' was highly conserved among mammalian type C and D oncoviruses. Point mutations used to inactivate SD' without changing the capsid-coding ability affected viral RNA splicing and reduced viral replication in infected cells.

Original language	English (US)
Pages (from-to)	3709-3714
Number of pages	6
Journal	Journal of virology
Volume	74
Issue number	8
DOIs	https://doi.org/10.1128/JVI.74.8.3709-3714.2000
State	Published - 2000

ASJC Scopus subject areas

Insect Science
Virology
Microbiology
Immunology

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10.1128/JVI.74.8.3709-3714.2000

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abstract = "Here we show the existence of a novel subgenomic 4.4-kb RNA in cells infected with the prototypic replication-competent Friend or Moloney murine leukemia viruses (MuLV). This RNA derives by splicing from an alternative donor site (SD') within the capsid-coding region to the canonical envelope splice acceptor site. The position and the sequence of SD' was highly conserved among mammalian type C and D oncoviruses. Point mutations used to inactivate SD' without changing the capsid-coding ability affected viral RNA splicing and reduced viral replication in infected cells.",

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T1 - A novel subgenomic murine leukemia virus RNA transcript results from alternative splicing

AU - Déjardin, Jérôme

AU - Bompard-Maréchal, Guillaume

AU - Audit, Muriel

AU - Hope, Thomas J.

AU - Sitbon, Marc

AU - Mougel, Marylène

PY - 2000

Y1 - 2000

N2 - Here we show the existence of a novel subgenomic 4.4-kb RNA in cells infected with the prototypic replication-competent Friend or Moloney murine leukemia viruses (MuLV). This RNA derives by splicing from an alternative donor site (SD') within the capsid-coding region to the canonical envelope splice acceptor site. The position and the sequence of SD' was highly conserved among mammalian type C and D oncoviruses. Point mutations used to inactivate SD' without changing the capsid-coding ability affected viral RNA splicing and reduced viral replication in infected cells.

AB - Here we show the existence of a novel subgenomic 4.4-kb RNA in cells infected with the prototypic replication-competent Friend or Moloney murine leukemia viruses (MuLV). This RNA derives by splicing from an alternative donor site (SD') within the capsid-coding region to the canonical envelope splice acceptor site. The position and the sequence of SD' was highly conserved among mammalian type C and D oncoviruses. Point mutations used to inactivate SD' without changing the capsid-coding ability affected viral RNA splicing and reduced viral replication in infected cells.

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JO - Journal of virology

JF - Journal of virology

IS - 8

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A novel subgenomic murine leukemia virus RNA transcript results from alternative splicing

Abstract

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