A novel yeast protein showing specific association with the cyclin-dependent kinase 5

Q. Q. Huang; K. Y. Lee; J. H. Wang

doi:10.1016/0014-5793(95)01394-6

A novel yeast protein showing specific association with the cyclin-dependent kinase 5

Q. Q. Huang^*, K. Y. Lee, J. H. Wang

^*Corresponding author for this work

Medicine, Rheumatology Division

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The present study describes a significant amino acid sequence homology between neuronal Cdk5 activator (nck5a) and an open reading frame of an unknown gene on the yeast S, cerevisiae chromosome III. A cDNA encoding a 25 kDa fragment of this yeast protein, the region containing homologous sequence to nck5a was cloned and expressed in E. coli as a glutathione-S-transferase fusion protein (GST-p25Y), GST-p25Y was found to block the in vitro activation of Cdk5 by nck5a and to affinity precipitate Cdk5 from bovine brain extract. The observations indicate that the yeast protein is capable of specific and high affinity association with Cdk5.

Original language	English (US)
Pages (from-to)	48-50
Number of pages	3
Journal	FEBS Letters
Volume	378
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(95)01394-6
State	Published - Jan 2 1996

Keywords

Cdk5
Neuronal Cdk5 activator (nck5a)
Yeast

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(95)01394-6

Cite this

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title = "A novel yeast protein showing specific association with the cyclin-dependent kinase 5",

abstract = "The present study describes a significant amino acid sequence homology between neuronal Cdk5 activator (nck5a) and an open reading frame of an unknown gene on the yeast S, cerevisiae chromosome III. A cDNA encoding a 25 kDa fragment of this yeast protein, the region containing homologous sequence to nck5a was cloned and expressed in E. coli as a glutathione-S-transferase fusion protein (GST-p25Y), GST-p25Y was found to block the in vitro activation of Cdk5 by nck5a and to affinity precipitate Cdk5 from bovine brain extract. The observations indicate that the yeast protein is capable of specific and high affinity association with Cdk5.",

keywords = "Cdk5, Neuronal Cdk5 activator (nck5a), Yeast",

author = "Huang, {Q. Q.} and Lee, {K. Y.} and Wang, {J. H.}",

note = "Funding Information: Acknowledgements: We thank Dr. Dallon Young for providing the yeast genomic DNA and yeast strains. This work was supported by operating grants from the Medical Research Council of Canada, the National Cancer Institute of Canada, and Alzheimer{\textquoteright}s Society of Canada.",

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AU - Lee, K. Y.

AU - Wang, J. H.

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N2 - The present study describes a significant amino acid sequence homology between neuronal Cdk5 activator (nck5a) and an open reading frame of an unknown gene on the yeast S, cerevisiae chromosome III. A cDNA encoding a 25 kDa fragment of this yeast protein, the region containing homologous sequence to nck5a was cloned and expressed in E. coli as a glutathione-S-transferase fusion protein (GST-p25Y), GST-p25Y was found to block the in vitro activation of Cdk5 by nck5a and to affinity precipitate Cdk5 from bovine brain extract. The observations indicate that the yeast protein is capable of specific and high affinity association with Cdk5.

AB - The present study describes a significant amino acid sequence homology between neuronal Cdk5 activator (nck5a) and an open reading frame of an unknown gene on the yeast S, cerevisiae chromosome III. A cDNA encoding a 25 kDa fragment of this yeast protein, the region containing homologous sequence to nck5a was cloned and expressed in E. coli as a glutathione-S-transferase fusion protein (GST-p25Y), GST-p25Y was found to block the in vitro activation of Cdk5 by nck5a and to affinity precipitate Cdk5 from bovine brain extract. The observations indicate that the yeast protein is capable of specific and high affinity association with Cdk5.

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KW - Neuronal Cdk5 activator (nck5a)

KW - Yeast

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A novel yeast protein showing specific association with the cyclin-dependent kinase 5

Abstract

Keywords

ASJC Scopus subject areas

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