A one-step cobalt-ferrocyanide method for histochemical demonstration of acetylcholinesterase activity in central nervous system tissue

C. R. Schatz, C. Geula*, R. J. Morecraft, M. M. Mesulam

*Corresponding author for this work

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We introduce a one-step histochemical method with cobalt as the precipitating agent for ferrocyanide for the light microscopic demonstration of acetylcholinesterase activity. This method was used to demonstrate acetylcholinesterase in normal cortical fibers and neurons, as well as pathological elements such as plaques and tangles. This procedure can also be easily combined with immunohistochemical methods that use diaminobenzidine as a chromogen.

Original languageEnglish (US)
Pages (from-to)431-434
Number of pages4
JournalJournal of Histochemistry and Cytochemistry
Volume40
Issue number3
DOIs
StatePublished - Jan 1 1992

Keywords

  • Acetylcholinesterase histochemistry
  • Cortical fibers
  • Human brain
  • Immunohistochemistry
  • Monkey brain
  • Plaques
  • Tangles

ASJC Scopus subject areas

  • Anatomy
  • Histology

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