A one-step ligation system for rapid generation of armed, conditionally-replicating adenoviruses

Xing Li; Ruiting Xia; Jing Zhao; Dongyang Wang; Qinwen Mao; Haibin Xia

doi:10.1007/s10529-013-1214-6

A one-step ligation system for rapid generation of armed, conditionally-replicating adenoviruses

Xing Li, Ruiting Xia, Jing Zhao, Dongyang Wang, Qinwen Mao, Haibin Xia^*

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

Abstract

Conditionally-replicating adenoviruses (CRAd) are becoming a promising strategy for cancer therapy. However, the generation of CRAd is complicated and time-consuming, especially for armed CRAd Ad5-D24. A one-step ligation system is now reported for rapid construction of an armed CRAd Ad5-D24 carrying two foreign transgenes. The system has the following features: (1) the CRAd backbone pCRAd5-D24-BS-Lacza contains two unique restriction sites between the fiber and E4 for easy introduction of a transgene expression cassette; and (2) a bi-directional promoter in pshuttle-BS-Dual allows for the expression of two independent transgenes. As proof of principle, Ad5-D24 CRAd carrying eGFP and mCherry was generated and examined. This novel ligation system will be a versatile and efficient tool in cancer viral gene therapy.

Original language	English (US)
Pages (from-to)	1215-1221
Number of pages	7
Journal	Biotechnology Letters
Volume	35
Issue number	8
DOIs	https://doi.org/10.1007/s10529-013-1214-6
State	Published - Aug 2013

Keywords

Adenovirus construction
Conditionally-replicating adenovirus
Gene therapy
Transgene

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s10529-013-1214-6

Cite this

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title = "A one-step ligation system for rapid generation of armed, conditionally-replicating adenoviruses",

abstract = "Conditionally-replicating adenoviruses (CRAd) are becoming a promising strategy for cancer therapy. However, the generation of CRAd is complicated and time-consuming, especially for armed CRAd Ad5-D24. A one-step ligation system is now reported for rapid construction of an armed CRAd Ad5-D24 carrying two foreign transgenes. The system has the following features: (1) the CRAd backbone pCRAd5-D24-BS-Lacza contains two unique restriction sites between the fiber and E4 for easy introduction of a transgene expression cassette; and (2) a bi-directional promoter in pshuttle-BS-Dual allows for the expression of two independent transgenes. As proof of principle, Ad5-D24 CRAd carrying eGFP and mCherry was generated and examined. This novel ligation system will be a versatile and efficient tool in cancer viral gene therapy.",

keywords = "Adenovirus construction, Conditionally-replicating adenovirus, Gene therapy, Transgene",

author = "Xing Li and Ruiting Xia and Jing Zhao and Dongyang Wang and Qinwen Mao and Haibin Xia",

note = "Funding Information: Acknowledgments This study was supported by the research grants to H.X. from the National Natural Science Foundation of China (Nos. 81272543 and 31070137).",

year = "2013",

month = aug,

doi = "10.1007/s10529-013-1214-6",

language = "English (US)",

volume = "35",

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journal = "Biotechnology Letters",

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T1 - A one-step ligation system for rapid generation of armed, conditionally-replicating adenoviruses

AU - Li, Xing

AU - Xia, Ruiting

AU - Zhao, Jing

AU - Wang, Dongyang

AU - Mao, Qinwen

AU - Xia, Haibin

N1 - Funding Information: Acknowledgments This study was supported by the research grants to H.X. from the National Natural Science Foundation of China (Nos. 81272543 and 31070137).

PY - 2013/8

Y1 - 2013/8

N2 - Conditionally-replicating adenoviruses (CRAd) are becoming a promising strategy for cancer therapy. However, the generation of CRAd is complicated and time-consuming, especially for armed CRAd Ad5-D24. A one-step ligation system is now reported for rapid construction of an armed CRAd Ad5-D24 carrying two foreign transgenes. The system has the following features: (1) the CRAd backbone pCRAd5-D24-BS-Lacza contains two unique restriction sites between the fiber and E4 for easy introduction of a transgene expression cassette; and (2) a bi-directional promoter in pshuttle-BS-Dual allows for the expression of two independent transgenes. As proof of principle, Ad5-D24 CRAd carrying eGFP and mCherry was generated and examined. This novel ligation system will be a versatile and efficient tool in cancer viral gene therapy.

AB - Conditionally-replicating adenoviruses (CRAd) are becoming a promising strategy for cancer therapy. However, the generation of CRAd is complicated and time-consuming, especially for armed CRAd Ad5-D24. A one-step ligation system is now reported for rapid construction of an armed CRAd Ad5-D24 carrying two foreign transgenes. The system has the following features: (1) the CRAd backbone pCRAd5-D24-BS-Lacza contains two unique restriction sites between the fiber and E4 for easy introduction of a transgene expression cassette; and (2) a bi-directional promoter in pshuttle-BS-Dual allows for the expression of two independent transgenes. As proof of principle, Ad5-D24 CRAd carrying eGFP and mCherry was generated and examined. This novel ligation system will be a versatile and efficient tool in cancer viral gene therapy.

KW - Adenovirus construction

KW - Conditionally-replicating adenovirus

KW - Gene therapy

KW - Transgene

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SN - 0141-5492

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JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 8

ER -

A one-step ligation system for rapid generation of armed, conditionally-replicating adenoviruses

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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