A one-step ligation system for rapid generation of armed, conditionally-replicating adenoviruses

Xing Li, Ruiting Xia, Jing Zhao, Dongyang Wang, Qinwen Mao, Haibin Xia*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Conditionally-replicating adenoviruses (CRAd) are becoming a promising strategy for cancer therapy. However, the generation of CRAd is complicated and time-consuming, especially for armed CRAd Ad5-D24. A one-step ligation system is now reported for rapid construction of an armed CRAd Ad5-D24 carrying two foreign transgenes. The system has the following features: (1) the CRAd backbone pCRAd5-D24-BS-Lacza contains two unique restriction sites between the fiber and E4 for easy introduction of a transgene expression cassette; and (2) a bi-directional promoter in pshuttle-BS-Dual allows for the expression of two independent transgenes. As proof of principle, Ad5-D24 CRAd carrying eGFP and mCherry was generated and examined. This novel ligation system will be a versatile and efficient tool in cancer viral gene therapy.

Original languageEnglish (US)
Pages (from-to)1215-1221
Number of pages7
JournalBiotechnology Letters
Volume35
Issue number8
DOIs
StatePublished - Aug 1 2013

Keywords

  • Adenovirus construction
  • Conditionally-replicating adenovirus
  • Gene therapy
  • Transgene

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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