Abstract
Calcineurin, a neuronally enriched, calcium-stimulated phosphatase, is an important modulator of many neuronal processes, including several that are physiologically related to the pathology of traumatic brain injury. The effect of moderate, central fluid percussion injury on the subcellular distribution of this important neuronal enzyme was examined. Animals were sacrificed at several time points post-injury and calcineurin distribution in subcellular fractions was assayed by Western blot analysis and immunohistochemistry. A persistent increase in calcineurin concentration was observed in crude synaptoplasmic membrane-containing fractions. In cortical fractions, calcineurin immunoreactivity remained persistently increased for 2 weeks post-injury. In hippocampal homogenates, calcineurin immunoreactivity remained increased for up to 4 weeks. Finally, immunohistochemical analysis of hippocampal slices revealed increased staining in the apical dendrites of CA1 neurons. The increased staining was greatest in magnitude 24 h post-injury; however, staining was still more intense than control 4 weeks post-injury. The data support the conclusion that fluid percussion injury results in redistribution of the enzyme in the rat forebrain. These changes have broad physiological implications, possibly resulting in altered cellular excitability or a greater likelihood of neuronal cell death.
Original language | English (US) |
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Pages (from-to) | 153-160 |
Number of pages | 8 |
Journal | Brain research |
Volume | 1048 |
Issue number | 1-2 |
DOIs | |
State | Published - Jun 28 2005 |
Funding
This work was supported by NIH grants P01-NS25630 (SBC), R01-NS399700 (SBC), and NIH predoctoral fellowship NSO7288 (JEK). The authors would like to thank Anh Lee and Matthew Ryan for their valuable technical assistance.
Keywords
- Hippocampus
- Immunohistochemistry
- Phosphatase
- Synaptoplasmic membrane
- Translocation
- Western analysis
ASJC Scopus subject areas
- General Neuroscience
- Molecular Biology
- Clinical Neurology
- Developmental Biology