@article{67b884044b124e37acaac3792bfd5f21,
title = "A Phenotypic Switch of Differentiated Glial Cells to Dedifferentiated Cells Is Regulated by Folate Receptor α",
abstract = "In a previous study, we showed that folate receptor-α (FRα) translocates to the nucleus where it acts as a transcription factor and upregulates Hes1, Oct4, Sox2, and Klf4 genes responsible for pluripotency. Here, we show that acetylation and phosphorylation of FRα favor its nuclear translocation in the presence of folate and can cause a phenotypic switch from differentiated glial cells to dedifferentiated cells. shRNA-FRα mediated knockdown of FRα was used to confirm the role of FRα in dedifferentiation. Ocimum sanctum hydrophilic fraction-1 treatment not only blocks the folate mediated dedifferentiation of glial cells but also promotes redifferentiation of dedifferentiated glial cells, possibly by reducing the nuclear translocation of ~38 kDa FRα and subsequent interaction with chromatin assembly factor-1. Stem Cells 2019;37:1441–1454.",
keywords = "Cranial neural crest cells, Dedifferentiation, Differentiation, Folate receptor alpha, Oct4, Sox2",
author = "Sarah Monick and Vineet Mohanty and Mariam Khan and Gowtham Yerneni and Raj Kumar and Jorge Cantu and Shunsuke Ichi and Guifa Xi and Singh, {Bal Ram} and Tadanori Tomita and Mayanil, {Chandra S K}",
note = "Funding Information: Thanks are due to Peggy A. Murphy and Barbara Mania-Farnell for critically reading the manuscript, and Dr. C. David James and Dr. John A. Kessler for very fruitful discussion. This work was supported in part by the State of Illinois Excellence in Academic Medicine award (C.S.M.); a grant from the Spastic Paralysis Research Foundation of Illinois-Eastern Iowa District of Kiwanis (C.S.M.); the Spina Bifida Association and Stanley Manne Children's Research Institute Pilot Grant award (C.S.M.); Eleanor Clarke Chair in Developmental Neurobiology (C.S.M.); and Neurofibromatosis fund from the Division of Pediatric Neurosurgery, Ann & Robert H. Lurie Children's Hospital of Chicago. We thank Bio-Rad for their generous gift of Trans-Blot Turbo and the ChemiDocMP system. Publisher Copyright: {\textcopyright} 2019 The Authors. Stem Cells published by Wiley Periodicals, Inc. on behalf of AlphaMed Press 2019",
year = "2019",
month = nov,
day = "1",
doi = "10.1002/stem.3067",
language = "English (US)",
volume = "37",
pages = "1441--1454",
journal = "Stem Cells",
issn = "1066-5099",
publisher = "AlphaMed Press",
number = "11",
}