A Phenotypic Switch of Differentiated Glial Cells to Dedifferentiated Cells Is Regulated by Folate Receptor α

Sarah Monick, Vineet Mohanty, Mariam Khan, Gowtham Yerneni, Raj Kumar, Jorge Cantu, Shunsuke Ichi, Guifa Xi, Bal Ram Singh, Tadanori Tomita, Chandra Shekhar Mayanil*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

In a previous study, we showed that folate receptor-α (FRα) translocates to the nucleus where it acts as a transcription factor and upregulates Hes1, Oct4, Sox2, and Klf4 genes responsible for pluripotency. Here, we show that acetylation and phosphorylation of FRα favor its nuclear translocation in the presence of folate and can cause a phenotypic switch from differentiated glial cells to dedifferentiated cells. shRNA-FRα mediated knockdown of FRα was used to confirm the role of FRα in dedifferentiation. Ocimum sanctum hydrophilic fraction-1 treatment not only blocks the folate mediated dedifferentiation of glial cells but also promotes redifferentiation of dedifferentiated glial cells, possibly by reducing the nuclear translocation of ~38 kDa FRα and subsequent interaction with chromatin assembly factor-1. Stem Cells 2019;37:1441–1454.

Original languageEnglish (US)
Pages (from-to)1441-1454
Number of pages14
JournalStem Cells
Volume37
Issue number11
DOIs
StatePublished - Nov 1 2019

Keywords

  • Cranial neural crest cells
  • Dedifferentiation
  • Differentiation
  • Folate receptor alpha
  • Oct4
  • Sox2

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

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