A photoclickable peptide microarray platform for facile and rapid screening of 3-D tissue microenvironments

Sadhana Sharma, Michael Floren, Ding Yonghui, Kurt R. Stenmark, Wei Tan, Stephanie J. Bryant*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Microarrays are powerful experimental tools for high-throughput screening of cellular behavior in multivariate microenvironments. Here, we present a new, facile and rapid screening method for probing cellular behavior in 3D tissue microenvironments. This method utilizes a photoclickable peptide microarray platform developed using electrospun fibrous poly(ethylene glycol) hydrogels and microarray contact printing. We investigated the utility of this platform with five different peptide motifs and ten cell types including stem, terminally differentiated, cancer or immune cells that were from either primary origin or cell lines and from different species. We validated the capabilities of this platform to screen arrays consisting of multiple peptide motifs and concentrations for selectivity to cellular adhesion and morphology. Moreover, this platform is amenable to controlled spatial presentation of peptides. We show that by leveraging the differential attachment affinities for two cell types to two different peptides, this platform can also be used to investigate cell-cell interactions through miniature co-culture peptide arrays. Our fibrous peptide microarray platform enables high-throughput screening of 3D tissue microenvironments in a facile and rapid manner to investigate cell-matrix interactions and cell-cell signaling and to identify optimal tissue microenvironments for cell-based therapies.

Original languageEnglish (US)
Pages (from-to)17-28
Number of pages12
JournalBiomaterials
Volume143
DOIs
StatePublished - Oct 2017

Keywords

  • Cell-cell interaction
  • Cell-matrix interaction
  • Fibrous hydrogel
  • High-throughput screening
  • Peptide microarray
  • Photoclickable
  • Tissue microenvironments

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Ceramics and Composites
  • Biomaterials
  • Mechanics of Materials

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