Abstract
A physical map of a genome is an essential guide for navigation, allowing the location of any gene or other landmark in the chromosomal DNA. We have constructed a physical map of the mouse genome that contains 296 contigs of overlapping bacterial clones and 16, 992 unique markers. The mouse contigs were aligned to the human genome sequence on the basis of 51, 486 homology matches, thus enabling use of the conserved synteny (correspondence between chromosome blocks) of the two genomes to accelerate construction of the mouse map. The map provides a framework for assembly of whole-genome shotgun sequence data, and a tile path of clones for generation of the reference sequence. Definition of the human-mouse alignment at this level of resolution enables identification of a mouse clone that corresponds to almost any position in the human genome. The human sequence may be used to facilitate construction of other mammalian genome maps using the same strategy.
Original language | English (US) |
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Pages (from-to) | 743-750 |
Number of pages | 8 |
Journal | Nature |
Volume | 418 |
Issue number | 6899 |
DOIs | |
State | Published - Aug 15 2002 |
Funding
The authors acknowledge the support of the Wellcome Trust, the National Institutes of Health and the US Department of Energy. We are grateful to the web team at the Sanger Institute for assistance with developing map displays, to P. Deloukas for RH map analysis, and E. Arnold-Berkowits, S. Lo, J. Gill and all present and past members of the Institute for Genomic Research BAC end sequencing team for the sequencing work.
ASJC Scopus subject areas
- General