TY - JOUR
T1 - A polyelectrolyte handle for single-molecule force spectroscopy
AU - Wang, Junpeng
AU - Kouznetsova, Tatiana B.
AU - Xia, Jianshe
AU - Ángeles, Felipe Jiménez
AU - de la Cruz, Monica Olvera
AU - Craig, Stephen L.
N1 - Funding Information:
This material is based on work supported by the Center for Molecularly Optimized Networks, National Science Foundation, under grant CHE‐2116298 and a Grant‐In‐Aid of Research from the National Academy of Sciences, administered by Sigma Xi, The Scientific Research Society (G20111015158063).
Funding Information:
Center for Molecularly Optimized Networks, National Science Foundation, Grant/Award Number: CHE‐2116298; Sigma Xi, The Scientific Research Society, Grant/Award Number: G20111015158063 Funding information
Publisher Copyright:
© 2023 Wiley Periodicals LLC.
PY - 2023
Y1 - 2023
N2 - Single-molecule force spectroscopy is a powerful tool for the quantitative investigation of the biophysics, polymer physics and mechanochemistry of individual polymer strands. One limitation of this technique is that the attachment between the tip of the atomic force microscope and the covalent or noncovalent analyte in a given pull is typically not strong enough to sustain the force at which the event of interest occurs, which makes the experiments time-consuming and inhibits throughput. Here we report a polyelectrolyte handle for single-molecule force spectroscopy that offers a combination of high (several hundred pN) attachment forces, good (~4%) success in obtaining a high-force (>200 pN) attachment, a non-fouling detachment process that allows for repetition, and specific attachment locations along the polymer analyte.
AB - Single-molecule force spectroscopy is a powerful tool for the quantitative investigation of the biophysics, polymer physics and mechanochemistry of individual polymer strands. One limitation of this technique is that the attachment between the tip of the atomic force microscope and the covalent or noncovalent analyte in a given pull is typically not strong enough to sustain the force at which the event of interest occurs, which makes the experiments time-consuming and inhibits throughput. Here we report a polyelectrolyte handle for single-molecule force spectroscopy that offers a combination of high (several hundred pN) attachment forces, good (~4%) success in obtaining a high-force (>200 pN) attachment, a non-fouling detachment process that allows for repetition, and specific attachment locations along the polymer analyte.
KW - atomic force microscope
KW - molecular dynamics simulations
KW - polymer physics
KW - polymer synthesis
KW - single molecule force spectroscopy
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U2 - 10.1002/pol.20230051
DO - 10.1002/pol.20230051
M3 - Article
AN - SCOPUS:85150513373
SN - 2642-4150
JO - Journal of Polymer Science
JF - Journal of Polymer Science
ER -