A postnatal peak in microglial development in the mouse hippocampus is correlated with heightened sensitivity to seizure triggers

Iris Kim, Lauren M. Mlsna, Stella Yoon, Brandy Le, Songtao Yu, Dan Xu, Sookyong Koh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

59 Scopus citations


Background: Explosive synaptogenesis and synaptic pruning occur in the hippocampus during the first two weeks of postnatal life, coincident with a heightened susceptibility to seizures in rodents. To determine the temporal correlation between microglial development and age-dependent susceptibility and response to seizures, we quantified developmental changes in basal microglia levels and seizure-induced microglial activation in the hippocampus of Cx3Cr1GFP/+ transgenic mice. Methods: Basal levels of microglia were quantified in the hippocampi of Cx3Cr1GFP/+ mice at P0, P5, P10, P15, P20, P25, P30, P40, and P60. Seizure susceptibility and seizure-induced microglial activation were assessed in response to febrile seizures (lipopolysaccharide followed by hyperthermia) and kainic acid-induced status epilepticus. Results: The density of microglia within the hippocampus increased rapidly after birth, reaching a peak during the second week of life - the age at which the animals became most vulnerable to seizure triggers. In addition, this peak of microglial development and seizure vulnerability during the second postnatal week represented the time of maximal seizure-induced microglia activation. Conclusions: Overreactive innate immunity mediated by activated microglia may exacerbate acute injury to neuronal synapses and contribute to the long-term epileptogenic effects of early-life seizures. Anti-inflammatory therapy targeting excessive production of inflammatory mediators by activated microglia, therefore, may be an effective age-specific therapeutic strategy to minimize neuronal dysfunction and prevent increases in susceptibility to subsequent seizures in developing animals.

Original languageEnglish (US)
Pages (from-to)1-4
Number of pages4
JournalBrain and Behavior
Issue number12
StatePublished - Dec 1 2015


  • Early life
  • Epilepsy
  • Febrile seizures
  • Status epilepticus

ASJC Scopus subject areas

  • Behavioral Neuroscience


Dive into the research topics of 'A postnatal peak in microglial development in the mouse hippocampus is correlated with heightened sensitivity to seizure triggers'. Together they form a unique fingerprint.

Cite this