A potassium channel in cultured chondrocytes

Micaela Grandolfo*, Marco Martina, Fabio Ruzzier, Franco Vittur

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Chondrocytes, obtained from preosseous cartilage, were studied by patch clamp technique in cell-attached recording configuration, and single potassium channels were characterized at different stages of culture. After 3 days, outward currents were present, with an open probability increasing with depolarization, and the K+ channels showing a mean slope conductance of 82 pS in asymmetric and 168 pS in symmetric potassium solution. Tetraethylammonium (TEA) and quinidine blocked the channels. Cells at confluence showed similar channel activity, with conductances of 121 and 252 pS, respectively. We suggest that culture time and/or conditions may modify K+ channels or induce the expression of a new type of channels.

Original languageEnglish (US)
Pages (from-to)302-307
Number of pages6
JournalCalcified Tissue International
Volume47
Issue number5
DOIs
StatePublished - Nov 1 1990

Keywords

  • Chondrocytes
  • Patch clamp
  • Postassium channels

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

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