A potassium channel in cultured chondrocytes

Micaela Grandolfo; Marco Martina; Fabio Ruzzier; Franco Vittur

doi:10.1007/BF02555913

A potassium channel in cultured chondrocytes

Micaela Grandolfo^*, Marco Martina, Fabio Ruzzier, Franco Vittur

^*Corresponding author for this work

Neuroscience

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Chondrocytes, obtained from preosseous cartilage, were studied by patch clamp technique in cell-attached recording configuration, and single potassium channels were characterized at different stages of culture. After 3 days, outward currents were present, with an open probability increasing with depolarization, and the K⁺ channels showing a mean slope conductance of 82 pS in asymmetric and 168 pS in symmetric potassium solution. Tetraethylammonium (TEA) and quinidine blocked the channels. Cells at confluence showed similar channel activity, with conductances of 121 and 252 pS, respectively. We suggest that culture time and/or conditions may modify K⁺ channels or induce the expression of a new type of channels.

Original language	English (US)
Pages (from-to)	302-307
Number of pages	6
Journal	Calcified Tissue International
Volume	47
Issue number	5
DOIs	https://doi.org/10.1007/BF02555913
State	Published - Nov 1 1990

Keywords

Chondrocytes
Patch clamp
Postassium channels

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Orthopedics and Sports Medicine
Endocrinology

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10.1007/BF02555913

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title = "A potassium channel in cultured chondrocytes",

abstract = "Chondrocytes, obtained from preosseous cartilage, were studied by patch clamp technique in cell-attached recording configuration, and single potassium channels were characterized at different stages of culture. After 3 days, outward currents were present, with an open probability increasing with depolarization, and the K+ channels showing a mean slope conductance of 82 pS in asymmetric and 168 pS in symmetric potassium solution. Tetraethylammonium (TEA) and quinidine blocked the channels. Cells at confluence showed similar channel activity, with conductances of 121 and 252 pS, respectively. We suggest that culture time and/or conditions may modify K+ channels or induce the expression of a new type of channels.",

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T1 - A potassium channel in cultured chondrocytes

AU - Grandolfo, Micaela

AU - Martina, Marco

AU - Ruzzier, Fabio

AU - Vittur, Franco

PY - 1990/11/1

Y1 - 1990/11/1

N2 - Chondrocytes, obtained from preosseous cartilage, were studied by patch clamp technique in cell-attached recording configuration, and single potassium channels were characterized at different stages of culture. After 3 days, outward currents were present, with an open probability increasing with depolarization, and the K+ channels showing a mean slope conductance of 82 pS in asymmetric and 168 pS in symmetric potassium solution. Tetraethylammonium (TEA) and quinidine blocked the channels. Cells at confluence showed similar channel activity, with conductances of 121 and 252 pS, respectively. We suggest that culture time and/or conditions may modify K+ channels or induce the expression of a new type of channels.

AB - Chondrocytes, obtained from preosseous cartilage, were studied by patch clamp technique in cell-attached recording configuration, and single potassium channels were characterized at different stages of culture. After 3 days, outward currents were present, with an open probability increasing with depolarization, and the K+ channels showing a mean slope conductance of 82 pS in asymmetric and 168 pS in symmetric potassium solution. Tetraethylammonium (TEA) and quinidine blocked the channels. Cells at confluence showed similar channel activity, with conductances of 121 and 252 pS, respectively. We suggest that culture time and/or conditions may modify K+ channels or induce the expression of a new type of channels.

KW - Chondrocytes

KW - Patch clamp

KW - Postassium channels

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A potassium channel in cultured chondrocytes

Abstract

Keywords

ASJC Scopus subject areas

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