A pressure system for the microinjection of substances into the brain of awake monkeys

Elisa C. Dias; Mark A. Segraves

doi:10.1016/S0165-0270(96)00154-9

A pressure system for the microinjection of substances into the brain of awake monkeys

Elisa C. Dias, Mark A. Segraves^*

^*Corresponding author for this work

Neurobiology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

We describe a method for placing pressure microinjections of drugs or anatomical tracers in physiologically defined sites in the brain of awake monkeys. This method provides a means to record neuronal activity from the tip of an injection cannula so that an injection can be made at a physiologically defined location. It uses pressure in a closed system to precisely control the amount of fluid injected and provides a visible means for monitoring injection volume. The injection cannula is easy to make with readily available components and can be used repeatedly for multiple recording sessions and injections.

Original language	English (US)
Pages (from-to)	43-47
Number of pages	5
Journal	Journal of Neuroscience Methods
Volume	72
Issue number	1
DOIs	https://doi.org/10.1016/S0165-0270(96)00154-9
State	Published - Mar 1997

Funding

We are grateful to the staff of Northwestern's Instrument Shop for machining. We thank Deanne Compaan and Janet Helminski for their comments on the manuscript. Supported by NIH Grants EY08212 and EY06716.

Keywords

Awake behaving primate
Microinjection
Neurophysiology

ASJC Scopus subject areas

General Neuroscience

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10.1016/S0165-0270(96)00154-9

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abstract = "We describe a method for placing pressure microinjections of drugs or anatomical tracers in physiologically defined sites in the brain of awake monkeys. This method provides a means to record neuronal activity from the tip of an injection cannula so that an injection can be made at a physiologically defined location. It uses pressure in a closed system to precisely control the amount of fluid injected and provides a visible means for monitoring injection volume. The injection cannula is easy to make with readily available components and can be used repeatedly for multiple recording sessions and injections.",

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AU - Segraves, Mark A.

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N2 - We describe a method for placing pressure microinjections of drugs or anatomical tracers in physiologically defined sites in the brain of awake monkeys. This method provides a means to record neuronal activity from the tip of an injection cannula so that an injection can be made at a physiologically defined location. It uses pressure in a closed system to precisely control the amount of fluid injected and provides a visible means for monitoring injection volume. The injection cannula is easy to make with readily available components and can be used repeatedly for multiple recording sessions and injections.

AB - We describe a method for placing pressure microinjections of drugs or anatomical tracers in physiologically defined sites in the brain of awake monkeys. This method provides a means to record neuronal activity from the tip of an injection cannula so that an injection can be made at a physiologically defined location. It uses pressure in a closed system to precisely control the amount of fluid injected and provides a visible means for monitoring injection volume. The injection cannula is easy to make with readily available components and can be used repeatedly for multiple recording sessions and injections.

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KW - Microinjection

KW - Neurophysiology

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A pressure system for the microinjection of substances into the brain of awake monkeys

Abstract

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Keywords

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