A primary encephalocele culture yields a pure population of human astrocytes

Ron L. Alterman; Richard S. Morrison; James T. Goodrich; Peter Papenhausen; Joseph R. Moskal

doi:10.1016/0006-8993(91)91334-W

A primary encephalocele culture yields a pure population of human astrocytes

Ron L. Alterman^*, Richard S. Morrison, James T. Goodrich, Peter Papenhausen, Joseph R. Moskal

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

In order to develop a reliable source of human astrocytes for in vitro studies, we established a primary explant culture of a human encephalocele. This culture yielded a population of cells which were karyotypically normal, morphologically resembled astrocytes, expressed glial fibrillary acid protein, and responded mitogenically to exogenous growth factors. We conclude that white matter derived from human encephaloceles can be used to generate pure populations of normal astrocytes.

Original language	English (US)
Pages (from-to)	319-323
Number of pages	5
Journal	Brain research
Volume	550
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(91)91334-W
State	Published - Jun 7 1991

Keywords

Astrocyte
Encephalocele
Glia cell culture
Glial fibrillary acid protein
Growth factor

ASJC Scopus subject areas

Clinical Neurology
Molecular Biology
General Neuroscience
Developmental Biology

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10.1016/0006-8993(91)91334-W

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title = "A primary encephalocele culture yields a pure population of human astrocytes",

abstract = "In order to develop a reliable source of human astrocytes for in vitro studies, we established a primary explant culture of a human encephalocele. This culture yielded a population of cells which were karyotypically normal, morphologically resembled astrocytes, expressed glial fibrillary acid protein, and responded mitogenically to exogenous growth factors. We conclude that white matter derived from human encephaloceles can be used to generate pure populations of normal astrocytes.",

keywords = "Astrocyte, Encephalocele, Glia cell culture, Glial fibrillary acid protein, Growth factor",

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AU - Alterman, Ron L.

AU - Morrison, Richard S.

AU - Goodrich, James T.

AU - Papenhausen, Peter

AU - Moskal, Joseph R.

PY - 1991/6/7

Y1 - 1991/6/7

N2 - In order to develop a reliable source of human astrocytes for in vitro studies, we established a primary explant culture of a human encephalocele. This culture yielded a population of cells which were karyotypically normal, morphologically resembled astrocytes, expressed glial fibrillary acid protein, and responded mitogenically to exogenous growth factors. We conclude that white matter derived from human encephaloceles can be used to generate pure populations of normal astrocytes.

AB - In order to develop a reliable source of human astrocytes for in vitro studies, we established a primary explant culture of a human encephalocele. This culture yielded a population of cells which were karyotypically normal, morphologically resembled astrocytes, expressed glial fibrillary acid protein, and responded mitogenically to exogenous growth factors. We conclude that white matter derived from human encephaloceles can be used to generate pure populations of normal astrocytes.

KW - Astrocyte

KW - Encephalocele

KW - Glia cell culture

KW - Glial fibrillary acid protein

KW - Growth factor

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A primary encephalocele culture yields a pure population of human astrocytes

Abstract

Keywords

ASJC Scopus subject areas

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