A promoter methylation pattern in the N-methyl-D-aspartate receptor 2B gene predicts poor prognosis in esophageal squamous cell carcinoma

Sook Kim Myoung, Keishi Yamashita, Kwang Chae Young, Yutaka Tokumaru, Xiaofei Chang, Marianna Zahurak, Motonobu Osada, Hannah Lui Park, Alice Chuang, Joseph A. Califano, David Sidransky*

*Corresponding author for this work

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Purpose: To investigate whether the promoter methylation pattern in N-methyl-D-aspartate receptor 2B (NMDAR2B) is correlated with clinical features of human esophageal squamous cell carcinoma (ESCC), the methylation status of the gene was examined at three different sites (P1, P2, and P3) where two CpG islands reside within 1kb upstream of the transcription start site. Experimental Design: Three independent modalities for methylation analysis (bisulfite sequencing, combined bisulfite restriction analysis, and TaqMan methylation-specific PCR) were done to analyze total 67 ESCC tissues that included 43 primary tumors with well-characterized clinicopathologic variables including patient outcome. Results: Using an optimized cutoff value based on quantitative methylation-specific PCR, we found that patients with higher NMDAR2B methylation ratio in the proximal region (P1) showed a worse 5-year disease-specific survival rate than those without NMDAR2B methylation (P < 0.006). A significant correlation was also seen between NMDAR2B promoter methylation and the presence of vascular permeation (P = 0.03). Conclusion: NMDAR2B promoter methylation could be a clinically applicable marker in ESCC.

Original languageEnglish (US)
Pages (from-to)6658-6665
Number of pages8
JournalClinical Cancer Research
Volume13
Issue number22
DOIs
StatePublished - Nov 15 2007

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Myoung, S. K., Yamashita, K., Young, K. C., Tokumaru, Y., Chang, X., Zahurak, M., Osada, M., Park, H. L., Chuang, A., Califano, J. A., & Sidransky, D. (2007). A promoter methylation pattern in the N-methyl-D-aspartate receptor 2B gene predicts poor prognosis in esophageal squamous cell carcinoma. Clinical Cancer Research, 13(22), 6658-6665. https://doi.org/10.1158/1078-0432.CCR-07-1178