Abstract
We determined HIV-1 pol gene sequences from self-collected rectal swabs of HIV-positive young men who have sex with men and transgender women. HIV-1 pol was amplified from 39/96 (41%) rectal swabs, including 29/77 (38%) prevalent and 10/19 (53%) incident HIV-1 infections (p < .001). Pol did not amplify from rectal swabs from participants with plasma viral load <1,000 copies/mL. Each rectal swab-derived amplicon consensus sequence was most closely related to the paired plasma virion RNA-derived sequence from the same participant. Results document a rectal mucosal source of HIV-1 in infected persons and suggest usefulness for noninvasive study of biological mechanisms underlying the epidemiologic risk to an insertive partner of HIV-1 acquisition during condomless anal sex.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 92-95 |
| Number of pages | 4 |
| Journal | AIDS research and human retroviruses |
| Volume | 36 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2020 |
Funding
This work was supported by grants from the National Institute on Drug Abuse (U01DA036939, PI: B.M.; F32DA046313, PI: E.M.) at the National Institutes of Health (NIH). This work also benefited from collaborative infrastructure enabled by the Third Coast Center for AIDS Research (CFAR), an NIH funded center (P30 AI117943).
Keywords
- HIV
- mucosal
- phylogenetics
ASJC Scopus subject areas
- Immunology
- Virology
- Infectious Diseases
