A prospective, multi-Institutional diagnostic trial to determine pathologist accuracy in estimation of percentage of malignant cells

Hollis Viray Dr., Kevin Li, Thomas A. Long, Patricia Vasalos, Julia A. Bridge Dr., Lawrence J Jennings, Kevin C. Halling, Meera Hameed Dr., David L. Rimm Dr.

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Context.-The fraction of malignant cells in tumor tissue submitted for tests of genetic alterations is a critical variable in testing accuracy. That fraction is currently determined by pathologist visual estimation of the percentage of malignant cells. Inaccuracy could lead to a false-negative test result. Objective.-To describe a prospective, multi-institutional study to determine pathologist estimation accuracy. Design.-Ten320 magnification images of hematoxylineosin-stained colon tissue specimens were sent as an educational component of the College of American Pathologists KRAS-B 2011 Survey. Data from 194 labs were analyzed and compared to a criterion standard with comprehensive manual nuclear counts. Results.-Survey responses indicated low interlaboratory precision of pathologist estimation, but mean estimates were fairly accurate. A total of 5 of the 10 cases assessed showed more than 10% of respondents overestimating in a manner that could lead to false-negative test results. Conclusions.-The significance of estimation errors resulting in molecular testing failures with implications for patient care is unknown, but the current study suggests false-negative test results may occur.

Original languageEnglish (US)
Pages (from-to)1545-1549
Number of pages5
JournalArchives of Pathology and Laboratory Medicine
Volume137
Issue number11
DOIs
StatePublished - Nov 1 2013

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Patient Care
Colon
Pathologists
Surveys and Questionnaires
Neoplasms

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

Viray Dr., Hollis ; Li, Kevin ; Long, Thomas A. ; Vasalos, Patricia ; Bridge Dr., Julia A. ; Jennings, Lawrence J ; Halling, Kevin C. ; Hameed Dr., Meera ; Rimm Dr., David L. / A prospective, multi-Institutional diagnostic trial to determine pathologist accuracy in estimation of percentage of malignant cells. In: Archives of Pathology and Laboratory Medicine. 2013 ; Vol. 137, No. 11. pp. 1545-1549.
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A prospective, multi-Institutional diagnostic trial to determine pathologist accuracy in estimation of percentage of malignant cells. / Viray Dr., Hollis; Li, Kevin; Long, Thomas A.; Vasalos, Patricia; Bridge Dr., Julia A.; Jennings, Lawrence J; Halling, Kevin C.; Hameed Dr., Meera; Rimm Dr., David L.

In: Archives of Pathology and Laboratory Medicine, Vol. 137, No. 11, 01.11.2013, p. 1545-1549.

Research output: Contribution to journalArticle

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