A rapid method for counting cell nuclei using a particle sizer/counter

Andy A. Lin; Thang Nguyen; William M. Miller

doi:10.1007/BF00159989

A rapid method for counting cell nuclei using a particle sizer/counter

Andy A. Lin^*, Thang Nguyen, William M. Miller

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

We report here an improved method for nuclei counting utilizing Triton-X 100 to reduce the size of cell debris, thereby allowing the use of a particle sizer/counter. Furthermore, nuclei are completely released within 30 seconds, as compared to 1 hour using hypotonic solution. The method is accurate above 0.3 × 10⁶ cells/mL.

Original language	English (US)
Pages (from-to)	153-156
Number of pages	4
Journal	Biotechnology Techniques
Volume	5
Issue number	2
DOIs	https://doi.org/10.1007/BF00159989
State	Published - Mar 1991
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Applied Microbiology and Biotechnology

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10.1007/BF00159989

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title = "A rapid method for counting cell nuclei using a particle sizer/counter",

abstract = "We report here an improved method for nuclei counting utilizing Triton-X 100 to reduce the size of cell debris, thereby allowing the use of a particle sizer/counter. Furthermore, nuclei are completely released within 30 seconds, as compared to 1 hour using hypotonic solution. The method is accurate above 0.3 × 106 cells/mL.",

author = "Lin, {Andy A.} and Thang Nguyen and Miller, {William M.}",

year = "1991",

month = mar,

doi = "10.1007/BF00159989",

language = "English (US)",

volume = "5",

pages = "153--156",

journal = "Biotechnology Techniques",

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publisher = "Springer Science and Business Media B.V.",

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T1 - A rapid method for counting cell nuclei using a particle sizer/counter

AU - Lin, Andy A.

AU - Nguyen, Thang

AU - Miller, William M.

PY - 1991/3

Y1 - 1991/3

N2 - We report here an improved method for nuclei counting utilizing Triton-X 100 to reduce the size of cell debris, thereby allowing the use of a particle sizer/counter. Furthermore, nuclei are completely released within 30 seconds, as compared to 1 hour using hypotonic solution. The method is accurate above 0.3 × 106 cells/mL.

AB - We report here an improved method for nuclei counting utilizing Triton-X 100 to reduce the size of cell debris, thereby allowing the use of a particle sizer/counter. Furthermore, nuclei are completely released within 30 seconds, as compared to 1 hour using hypotonic solution. The method is accurate above 0.3 × 106 cells/mL.

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UR - http://www.scopus.com/inward/citedby.url?scp=0001227238&partnerID=8YFLogxK

U2 - 10.1007/BF00159989

DO - 10.1007/BF00159989

M3 - Article

AN - SCOPUS:0001227238

SN - 0951-208X

VL - 5

SP - 153

EP - 156

JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 2

ER -

A rapid method for counting cell nuclei using a particle sizer/counter

Abstract

ASJC Scopus subject areas

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