A rapid method for counting cell nuclei using a particle sizer/counter

Andy A. Lin*, Thang Nguyen, William M. Miller

*Corresponding author for this work

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

We report here an improved method for nuclei counting utilizing Triton-X 100 to reduce the size of cell debris, thereby allowing the use of a particle sizer/counter. Furthermore, nuclei are completely released within 30 seconds, as compared to 1 hour using hypotonic solution. The method is accurate above 0.3 × 106 cells/mL.

Original languageEnglish (US)
Pages (from-to)153-156
Number of pages4
JournalBiotechnology Techniques
Volume5
Issue number2
DOIs
StatePublished - Mar 1 1991

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology

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