A rapid method for quantitation of antiviral antibodies

Jeffrey D. Peterson*, Jerry Y. Kim, Roger W. Melvold, Stephen D. Miller, Carl Waltenbaugh

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

This paper examines the parameters necessary for the efficient measurement of anti-Theiler's murine encephalomyelitis virus (TMEV) antibodies in an affinity-dependent manner using a variation of a solid-phase particle concentration fluorescence immunoassay (PCFIA). By allowing antibody to react with fluorochrome-labelled virus in fluid phase and subsequently capturing the resulting virus-antibody complexes with anti-immunoglobulin coated polystyrene particles (fluid-phase PCFIA), the present assay allows for both greater sensitivity, specificity and preservation of conformational viral epitopes than do solid-phase immunoassays. Fluid-phase PCFIA proved to be a more rapid quantitative assay than ELISA and significantly diminished non-specific binding by both untreated and heat-inactivated normal mouse sera. This methodology also allowed us to perform competition assays and to determine the dissociation kinetics of anti-viral antibody preparations, investigations which cannot generally be performed as solid-phase immunoassays. Thus fluid-phase PCFIA is a rapid and efficient immunoassay with excellent reproducibility and great versatility.

Original languageEnglish (US)
Pages (from-to)83-94
Number of pages12
JournalJournal of Immunological Methods
Volume119
Issue number1
DOIs
StatePublished - Apr 21 1989

Keywords

  • Fluorescence immunoassay
  • Particle concentration fluorescence immunoassay
  • Theiler's murine encephalomyelitis virus
  • Viral antibody

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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