TY - JOUR
T1 - A recently established murine model of nasal polyps demonstrates activation of B cells, as occurs in human nasal polyps
AU - Kim, Dong Young
AU - Lee, Sun Hye
AU - Carter, Roderick G.
AU - Kato, Atsushi
AU - Schleimer, Robert P.
AU - Cho, Seong H.
N1 - Funding Information:
This work was supported by National Institutes of Health grant 1K23AI110731 and American Heart Association grant 11SDG7590063 (S.H.C.), and National Institutes of Health grants R37HL068546, R01HL078860, and U19AI106683, and funds from the Ernest Bazley Foundation (R.P.S.), and from the Research Resettlement Fund for the new faculty of Seoul National University (D.-Y.K.)
Publisher Copyright:
Copyright © 2016 by the American Thoracic Society.
PY - 2016/8
Y1 - 2016/8
N2 - Animal model systems are invaluable for examining human diseases. Our laboratory recently established a mouse model of nasal polyps (NPs) and investigated similarities and differences between this mouse model and human NPs. We especially focus on the hypothesis that B cell activation occurs during NP generation in the murine model. After induction of ovalbumin-induced allergic rhinosinusitis, 6% ovalbumin and Staphylococcus aureus enterotoxin B (10 ng) were instilled into the nasal cavity of mice three times per week for 8 weeks. The development of structures that somewhat resemble NPs (which we will refer to as NPs) was confirmed by hematoxylin and eosin staining. The mRNA and protein levels of various inflammatory cellmarkers andmediators were measured by real-time PCR in nasal tissue and by ELISA in nasal lavage fluid (NLF), respectively. Total Ig isotype levels in NLF were also quantitated using theMouse Ig IsotypingMultiplex kit (EMD Millipore, Billerica, MA) on a Luminex 200 instrument (Life Technologies, Grand Island, NY). Similar to human NPs, there were significant increases in gene expression of inflammatory cell markers, such as CD19, CD138, CD11c, and mast cell protease-6 in nasal tissue samples of the NP group compared with those of the control group. In further investigations of B cell activation, mRNA expressions of B cell activating factor and a proliferation-inducing ligandwere found to be significantly increased in mouse NP tissue. B cell-activating factor protein concentration and IgA and IgG1 levels in NLF were significantly higher in the NP group compared with the control group. In this study, the NP mousemodel demonstrated enhanced B cell responses, which are reminiscent of B cell responses in human NPs.
AB - Animal model systems are invaluable for examining human diseases. Our laboratory recently established a mouse model of nasal polyps (NPs) and investigated similarities and differences between this mouse model and human NPs. We especially focus on the hypothesis that B cell activation occurs during NP generation in the murine model. After induction of ovalbumin-induced allergic rhinosinusitis, 6% ovalbumin and Staphylococcus aureus enterotoxin B (10 ng) were instilled into the nasal cavity of mice three times per week for 8 weeks. The development of structures that somewhat resemble NPs (which we will refer to as NPs) was confirmed by hematoxylin and eosin staining. The mRNA and protein levels of various inflammatory cellmarkers andmediators were measured by real-time PCR in nasal tissue and by ELISA in nasal lavage fluid (NLF), respectively. Total Ig isotype levels in NLF were also quantitated using theMouse Ig IsotypingMultiplex kit (EMD Millipore, Billerica, MA) on a Luminex 200 instrument (Life Technologies, Grand Island, NY). Similar to human NPs, there were significant increases in gene expression of inflammatory cell markers, such as CD19, CD138, CD11c, and mast cell protease-6 in nasal tissue samples of the NP group compared with those of the control group. In further investigations of B cell activation, mRNA expressions of B cell activating factor and a proliferation-inducing ligandwere found to be significantly increased in mouse NP tissue. B cell-activating factor protein concentration and IgA and IgG1 levels in NLF were significantly higher in the NP group compared with the control group. In this study, the NP mousemodel demonstrated enhanced B cell responses, which are reminiscent of B cell responses in human NPs.
KW - Animal model; antibodies; B cells; chronic rhinosinusitis; nasal polyps
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U2 - 10.1165/rcmb.2016-0002RC
DO - 10.1165/rcmb.2016-0002RC
M3 - Article
C2 - 27163839
AN - SCOPUS:84988940565
SN - 1044-1549
VL - 55
SP - 170
EP - 175
JO - American Journal of Respiratory Cell and Molecular Biology
JF - American Journal of Respiratory Cell and Molecular Biology
IS - 2
ER -
