A recently established murine model of nasal polyps demonstrates activation of B cells, as occurs in human nasal polyps

Dong Young Kim, Sun Hye Lee, Roderick G. Carter, Atsushi Kato, Robert P. Schleimer, Seong H. Cho*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Animal model systems are invaluable for examining human diseases. Our laboratory recently established a mouse model of nasal polyps (NPs) and investigated similarities and differences between this mouse model and human NPs. We especially focus on the hypothesis that B cell activation occurs during NP generation in the murine model. After induction of ovalbumin-induced allergic rhinosinusitis, 6% ovalbumin and Staphylococcus aureus enterotoxin B (10 ng) were instilled into the nasal cavity of mice three times per week for 8 weeks. The development of structures that somewhat resemble NPs (which we will refer to as NPs) was confirmed by hematoxylin and eosin staining. The mRNA and protein levels of various inflammatory cellmarkers andmediators were measured by real-time PCR in nasal tissue and by ELISA in nasal lavage fluid (NLF), respectively. Total Ig isotype levels in NLF were also quantitated using theMouse Ig IsotypingMultiplex kit (EMD Millipore, Billerica, MA) on a Luminex 200 instrument (Life Technologies, Grand Island, NY). Similar to human NPs, there were significant increases in gene expression of inflammatory cell markers, such as CD19, CD138, CD11c, and mast cell protease-6 in nasal tissue samples of the NP group compared with those of the control group. In further investigations of B cell activation, mRNA expressions of B cell activating factor and a proliferation-inducing ligandwere found to be significantly increased in mouse NP tissue. B cell-activating factor protein concentration and IgA and IgG1 levels in NLF were significantly higher in the NP group compared with the control group. In this study, the NP mousemodel demonstrated enhanced B cell responses, which are reminiscent of B cell responses in human NPs.

Original languageEnglish (US)
Pages (from-to)170-175
Number of pages6
JournalAmerican journal of respiratory cell and molecular biology
Issue number2
StatePublished - Aug 2016


  • Animal model; antibodies; B cells; chronic rhinosinusitis; nasal polyps

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology


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