A receptor tyrosine kinase, UFO/Axl, and other genes isolated by a modified differential display PCR are overexpressed in metastatic prostatic carcinoma cell line DU145

A. N K Jacob, J. Kalapurakal, W. R. Davidson, G. Kandpal, N. Dunson, Y. Prashar, R. P. Kandpal*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

We have used a modified differential display PCR protocol for isolating 3' restriction fragments of cDNAs specifically expressed or overexpressed in metastatic prostate carcinoma cell line DU145. Several cDNA fragments were identified that matched to milk fat globule protein, UFO/Axl, a receptor tyrosine kinase, human homologue of a Xenopus maternal transcript, laminin and laminin receptor, human carcinoma-associated antigen, and some expressed sequence tags. The transcript for milk fat globule protein, a marker protein shown to be overexpressed in breast tumors, was elevated in DU145 cells. The expression of UFO/Axl, a receptor tyrosine kinase, was considerably higher in DU145 cells as compared to normal prostate cells and prostatic carcinoma cell line PC-3. The overexpression of UFO oncogene in DU145 cells is discussed in the context of prostate cancer metastasis.

Original languageEnglish (US)
Pages (from-to)325-332
Number of pages8
JournalCancer detection and prevention
Volume23
Issue number4
DOIs
StatePublished - 1999

Keywords

  • Differential display
  • Metastasis
  • Oncogene
  • Prostate
  • Receptor tyrosine kinase
  • UFO/Axl

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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