A region in domain II of the urokinase receptor required for urokinase binding

Khalil Bdeir, Alice Kuo, Andrew Mazar, Bruce S. Sachais, Weizhong Xiao, Susan Gawlak, Scott Harris, Abd Al Roof Higazi, Douglas B. Cines*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

The urokinase receptor is composed of three homologous domains based on disulfide spacing. The contribution of each domain to the binding and activation of single chain urokinase (scuPA) remains poorly understood. In the present paper we examined the role of domain II (DII) in these processes. Repositioning DII to the amino or carboxyl terminus of the molecule abolished binding of scuPA as did deleting the domain entirely. By using alanine-scanning mutagenesis, we identified a 9-amino acid continuous sequence in DII (Arg137-Arg145) required for both activities. Competition-inhibition and surface plasmon resonance studies demonstrated that mutation of Lys139 and His143 to alanine in soluble receptor (suPAR) reduced the affinity for scuPA ~5-fold due to an increase in the 'off rate.' Mutation of Arg137, Arg142, and Arg145, each to alanine, leads to an ~100-fold decrease in affinity attributable to a 10-fold decrease in the apparent 'on rate' and a 6-fold increase in off rate. These differences were confirmed on cells expressing variant urokinase receptor. suPARK139A/H143A displayed a 50% reduction in scuPA-mediated plasminogen activation activity, whereas the 3-arginine variant was unable to stimulate scuPA activity at all. Mutation of the three arginines did not affect binding of a decamer peptide antagonist of scuPA known to interact with DI and DIII. However, this mutation abolished both the binding of soluble DI to DII-III in the presence of scuPA and the synergistic activation of scuPA mediated by DI and wild type DII-DIII. These data show that DII is required for high affinity binding of scuPA and its activation. DII does not serve merely as a spacer function but appears to be required for interdomain cooperativity.

Original languageEnglish (US)
Pages (from-to)28532-28538
Number of pages7
JournalJournal of Biological Chemistry
Volume275
Issue number37
DOIs
StatePublished - Sep 15 2000

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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