A regional study of electrophoretically separated proteins from the crude synaptosomal fractions of the developing rat brain

Leonard G. Davis, Eric G. Brunngraber, Aryeh Routtenberg*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Albino rats of 1, 5, 9, 16, and 25 days of age were sacrificed, and six brain regions were dissected free. Crude synaptosomal preparations from each brain region and age were obtained by differential centrifugation. Protein content in these fractions from the six brain regions increased almost threefold between 1 and 25 days of age. The preparations were delipidated, solubilized, and subjected to polyacrylamide gel electrophoresis. The densitometric patterns of five samples from each age and brain region were analyzed for changes in the relative amount of stained protein bands. Of the polyacrylamide gel‐separated protein bands, 16 regional differences at specific ages and 20 developmental changes in specific brain regions were found to be statistically significant. The hippocampal region showed the greatest number of statistical changes in the protein pattern as a function of age, whereas the cerebellum showed none. The results suggest the importance of considering regional differences in studies of biochemical developmental changes.

Original languageEnglish (US)
Pages (from-to)25-34
Number of pages10
JournalJournal of Neuroscience Research
Volume4
Issue number1
DOIs
StatePublished - Jan 1 1979

Keywords

  • brain regions
  • development
  • hippocampus
  • polyacrylamide gels
  • synaptosomal fractions

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'A regional study of electrophoretically separated proteins from the crude synaptosomal fractions of the developing rat brain'. Together they form a unique fingerprint.

Cite this