A reliable method for obtaining high-resolution banded chromosome preparations from chorionic villus samples

Han Xiang Deng*, Lu Yun Li, Jia Hui Xia, Norio Niikawa

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

A reliable method for obtaining high-resolution banded chromosome preparations from chorionic villus samples (CVS) is described, in which a synchronization cell-culture technique with the use of thymidine is introduced. After culturing a suspensin of chorionic villus cells in a medium supplemented with 0.3 mg/ml thymidine for 13-17 hr, cells were washed twice, and recultured for further 8 hr. The cells were then treated with 10 μg/ml ethidium bromide or 1 μg/ml actinomycin D for 1 hr and subsequently with 0.01 μg/ml Colcemid for 30 min. The average mitotic index obtained was more than 60 mitoses/mg CVS, and more than 60% of mitoses proceeded to the prometaphase stage, the later prophase stage, or in between. The application of this method for prenatal diagnoses in five CVS in the first trimester pregnancies proved that it is a simple, practical and efficient prenatal diagnostic method.

Original languageEnglish (US)
Pages (from-to)189-193
Number of pages5
JournalThe Japanese Journal of Human Genetics
Volume35
Issue number2
DOIs
StatePublished - Jun 1990

Keywords

  • chroionic villi
  • high-resolution chromosome banding technique
  • prenatal diagnosis
  • synchronization cell-culture method

ASJC Scopus subject areas

  • Genetics(clinical)

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