A repetitive Dictyostelium gene family that is induced during differentiation and by heat shock

Charles Zuker; Joe Cappello; Rex L. Chisholm; Harvey F. Lodish

doi:10.1016/0092-8674(83)90557-3

A repetitive Dictyostelium gene family that is induced during differentiation and by heat shock

Charles Zuker^*, Joe Cappello, Rex L. Chisholm, Harvey F. Lodish

^*Corresponding author for this work

FSM Office for Research

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Clone pB41-6 (2.5 kb) contains sequences that are repeated 200-300 times in the Dictyostelium genome; about 40 of these sequences are part of a 4.5 kb repeated and apparently transposable genomic element. Clone pB41-6 hybridizes to a large number of cytoplasmic polyadenylated RNAs whose accumulation begins in the first hour of differentiation. In order to understand the regulation of these repeated sequences, we have sequenced pB41-6. It contains three long open reading frames in the "sense" strand. Remarkably, about 70 bases upstream of the transcription initiation site is a sequence identical to that responsible for induction of the Drosophila heat shock genes. A search of published sequences also generated a similar sequence upstream of one of the Dictyostelium actin genes. Indeed, we found that both pB41-6-related RNAs and actin mRNAs are increased as a result of heat shocking growing cells, and that transcription of pB41-6 sequences is induced by heat shock. Thus Dictyostelium contains a set of genes that are induced as a response to heat shock or to the stresses that trigger the initiation of development. We show here that the principal component of this "stress" is not amino acid starvation but the high density of the cells.

Original language	English (US)
Pages (from-to)	997-1005
Number of pages	9
Journal	Cell
Volume	34
Issue number	3
DOIs	https://doi.org/10.1016/0092-8674(83)90557-3
State	Published - Oct 1983

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/0092-8674(83)90557-3

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title = "A repetitive Dictyostelium gene family that is induced during differentiation and by heat shock",

abstract = "Clone pB41-6 (2.5 kb) contains sequences that are repeated 200-300 times in the Dictyostelium genome; about 40 of these sequences are part of a 4.5 kb repeated and apparently transposable genomic element. Clone pB41-6 hybridizes to a large number of cytoplasmic polyadenylated RNAs whose accumulation begins in the first hour of differentiation. In order to understand the regulation of these repeated sequences, we have sequenced pB41-6. It contains three long open reading frames in the {"}sense{"} strand. Remarkably, about 70 bases upstream of the transcription initiation site is a sequence identical to that responsible for induction of the Drosophila heat shock genes. A search of published sequences also generated a similar sequence upstream of one of the Dictyostelium actin genes. Indeed, we found that both pB41-6-related RNAs and actin mRNAs are increased as a result of heat shocking growing cells, and that transcription of pB41-6 sequences is induced by heat shock. Thus Dictyostelium contains a set of genes that are induced as a response to heat shock or to the stresses that trigger the initiation of development. We show here that the principal component of this {"}stress{"} is not amino acid starvation but the high density of the cells.",

author = "Charles Zuker and Joe Cappello and Chisholm, {Rex L.} and Lodish, {Harvey F.}",

note = "Funding Information: This work was supported by grant 79.00839.PCM from the NSF. We thank Miriam Boucher for her patience in the preparation of this manuscript, and Naomi Cohen for expert technical assistance. We also thank Mr. Jay Morgenstern for the backward-forwards sequencing. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to Indicate this fact.",

year = "1983",

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T1 - A repetitive Dictyostelium gene family that is induced during differentiation and by heat shock

AU - Zuker, Charles

AU - Cappello, Joe

AU - Chisholm, Rex L.

AU - Lodish, Harvey F.

N1 - Funding Information: This work was supported by grant 79.00839.PCM from the NSF. We thank Miriam Boucher for her patience in the preparation of this manuscript, and Naomi Cohen for expert technical assistance. We also thank Mr. Jay Morgenstern for the backward-forwards sequencing. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to Indicate this fact.

PY - 1983/10

Y1 - 1983/10

N2 - Clone pB41-6 (2.5 kb) contains sequences that are repeated 200-300 times in the Dictyostelium genome; about 40 of these sequences are part of a 4.5 kb repeated and apparently transposable genomic element. Clone pB41-6 hybridizes to a large number of cytoplasmic polyadenylated RNAs whose accumulation begins in the first hour of differentiation. In order to understand the regulation of these repeated sequences, we have sequenced pB41-6. It contains three long open reading frames in the "sense" strand. Remarkably, about 70 bases upstream of the transcription initiation site is a sequence identical to that responsible for induction of the Drosophila heat shock genes. A search of published sequences also generated a similar sequence upstream of one of the Dictyostelium actin genes. Indeed, we found that both pB41-6-related RNAs and actin mRNAs are increased as a result of heat shocking growing cells, and that transcription of pB41-6 sequences is induced by heat shock. Thus Dictyostelium contains a set of genes that are induced as a response to heat shock or to the stresses that trigger the initiation of development. We show here that the principal component of this "stress" is not amino acid starvation but the high density of the cells.

AB - Clone pB41-6 (2.5 kb) contains sequences that are repeated 200-300 times in the Dictyostelium genome; about 40 of these sequences are part of a 4.5 kb repeated and apparently transposable genomic element. Clone pB41-6 hybridizes to a large number of cytoplasmic polyadenylated RNAs whose accumulation begins in the first hour of differentiation. In order to understand the regulation of these repeated sequences, we have sequenced pB41-6. It contains three long open reading frames in the "sense" strand. Remarkably, about 70 bases upstream of the transcription initiation site is a sequence identical to that responsible for induction of the Drosophila heat shock genes. A search of published sequences also generated a similar sequence upstream of one of the Dictyostelium actin genes. Indeed, we found that both pB41-6-related RNAs and actin mRNAs are increased as a result of heat shocking growing cells, and that transcription of pB41-6 sequences is induced by heat shock. Thus Dictyostelium contains a set of genes that are induced as a response to heat shock or to the stresses that trigger the initiation of development. We show here that the principal component of this "stress" is not amino acid starvation but the high density of the cells.

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A repetitive Dictyostelium gene family that is induced during differentiation and by heat shock

Abstract

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