TY - JOUR
T1 - A resource for the simultaneous high-resolution mapping of multiple quantitative trait loci in rats:The NIH heterogeneous stock
AU - Johannesson, Martina
AU - Lopez-Aumatell, Regina
AU - Stridh, Pernilla
AU - Diez, Margarita
AU - Tuncel, Jonatan
AU - Blázquez, Gloria
AU - Martinez-Membrives, Esther
AU - Cañete, Toni
AU - Vicens-Costa, Elia
AU - Graham, Delyth
AU - Copley, Richard R.
AU - Hernandez-Pliego, Polinka
AU - Beyeen, Amennai D.
AU - Öckinger, Johan
AU - Fernández-Santamaría, Cristina
AU - Gulko, Percio S.
AU - Brenner, Max
AU - Tobeña, Adolf
AU - Guitart-Masip, Marc
AU - Giménez-Llort, Lydia
AU - Dominiczak, Anna
AU - Holmdahl, Rikard
AU - Gauguier, Dominique
AU - Olsson, Tomas
AU - Mott, Richard
AU - Valdar, William
AU - Redei, Eva E.
AU - Fernández-Teruel, Alberto
AU - Flint, Jonathan
PY - 2009/1
Y1 - 2009/1
N2 - The laboratory rat (Rattus norvegicus) is a key tool for the study of medicine and pharmacology for human health. A large database of phenotypes for integrated fields such as cardiovascular, neuroscience, and exercise physiology exists in the literature. However, the molecular characterization of the genetic loci that give rise to variation in these traits has proven to be difficult. Here we show how one obstacle to progress, the fine-mapping of quantitative trait loci (QTL), can be overcome by using an outbred population of rats. By use of a genetically heterogeneous stock of rats, we map a locus contributing to variation in a fear-related measure (two-way active avoidance in the shuttle box) to a region on chromosome 5 containing nine genes. By establishing a protocol measuring multiple phenotypes including immunology, neuroinflammation, and hematology, as well as cardiovascular, metabolic, and behavioral traits, we establish the rat HS as a new resource for the fine-mapping of QTLs contributing to variation in complex traits of biomedical relevance.
AB - The laboratory rat (Rattus norvegicus) is a key tool for the study of medicine and pharmacology for human health. A large database of phenotypes for integrated fields such as cardiovascular, neuroscience, and exercise physiology exists in the literature. However, the molecular characterization of the genetic loci that give rise to variation in these traits has proven to be difficult. Here we show how one obstacle to progress, the fine-mapping of quantitative trait loci (QTL), can be overcome by using an outbred population of rats. By use of a genetically heterogeneous stock of rats, we map a locus contributing to variation in a fear-related measure (two-way active avoidance in the shuttle box) to a region on chromosome 5 containing nine genes. By establishing a protocol measuring multiple phenotypes including immunology, neuroinflammation, and hematology, as well as cardiovascular, metabolic, and behavioral traits, we establish the rat HS as a new resource for the fine-mapping of QTLs contributing to variation in complex traits of biomedical relevance.
UR - http://www.scopus.com/inward/record.url?scp=61449224751&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=61449224751&partnerID=8YFLogxK
U2 - 10.1101/gr.081497.108
DO - 10.1101/gr.081497.108
M3 - Article
C2 - 18971309
AN - SCOPUS:61449224751
SN - 1088-9051
VL - 19
SP - 150
EP - 158
JO - Genome research
JF - Genome research
IS - 1
ER -