Abstract
The methods for the culture and cardiomyocyte differentiation of human embryonic stem cells, and later human induced pluripotent stem cells (hiPSC), have moved from a complex and uncontrolled systems to simplified and relatively robust protocols, using the knowledge and cues gathered at each step. HiPSC-derived cardiomyocytes have proven to be a useful tool in human disease modelling, drug discovery, developmental biology, and regenerative medicine. In this protocol review, we will highlight the evolution of protocols associated with hPSC culture, cardiomyocyte differentiation, sub-type specification, and cardiomyocyte maturation. We also discuss protocols for somatic cell direct reprogramming to cardiomyocyte-like cells.
Original language | English (US) |
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Article number | 101560 |
Journal | STAR Protocols |
Volume | 3 |
Issue number | 3 |
DOIs | |
State | Published - Sep 16 2022 |
Funding
This work was supported by NIH grants CA220002 and CA261898 (to P.W.B), R01 HL153220 (to Y.Z.); American Heart Association Postdoctoral Fellowship 874276 (to D.M.L-L.); the German Federal Ministry of Education and Research (BMBF)/German Center for Cardiovascular Research (DZHK) (to L.C.); the German Research Foundation (DFG) EXC 2067/1–390729940, CY 90/1-1 and SFB1002 S01 (to L.C.); and the Else-Kröner-Fersenius Foundation 2019_A75 (to L.C.). Figures were produced with the aid of SMART Servier Medical Art (www.smart.servier.com, Attribution 3.0 Unported (CC BY 3.0)), and Marcel Tisch via bioicons.com (CC0 1.0 Universal (CC0 1.0) Public Domain Dedication). This work was supported by NIH grants CA220002 and CA261898 (to P.W.B), R01 HL153220 (to Y.Z.); American Heart Association Postdoctoral Fellowship 874276 (to D.M.L-L.); the German Federal Ministry of Education and Research (BMBF)/ German Center for Cardiovascular Research (DZHK) (to L.C.); the German Research Foundation (DFG) EXC 2067/1–390729940 , CY 90/1-1 and SFB1002 S01 (to L.C.); and the Else-Kröner-Fersenius Foundation 2019_A75 (to L.C.). Figures were produced with the aid of SMART Servier Medical Art ( www.smart.servier.com , Attribution 3.0 Unported (CC BY 3.0)), and Marcel Tisch via bioicons.com (CC0 1.0 Universal (CC0 1.0) Public Domain Dedication).
Keywords
- Cell Differentiation
- Cell culture
- Stem Cells
ASJC Scopus subject areas
- General Immunology and Microbiology
- General Biochemistry, Genetics and Molecular Biology
- General Neuroscience