Abstract
Micropost-based microfluidic devices are widely used for microvascular network (MVN) formation in diverse research fields. However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key characteristics of MVN formation is investigated and a robust two-step seeding strategy to generate perfusable physiological MVNs in microfluidic devices is established.
| Original language | English (US) |
|---|---|
| Article number | 2200143 |
| Journal | Small Methods |
| Volume | 6 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 30 2022 |
Funding
Z.W. was supported by a Ludwig Center Fund Post‐Doctoral Research Fellowship from the Koch Institute for Integrative Cancer Research and by National Cancer Institute (U01 CA214381). S.E.S. was supported by a fellowship from the National Cancer Institute (K00CA212227). This work was supported by a National Cancer Institute Physical Sciences‐Oncology Network supplement to award U01 CA214381. The monocytes were kindly provided by Prof. Byran Bryson. R.D.K. is the co‐founder of and holds a significant financial interest in AIM Biotech, a company that produces microfluidic devices. R.D.K. also receives research support from Amgen, Novartis, Boehringer Ingelheim, GSK, AbbVie and Roche. D.A.B. is a co‐founder of and holds a significant financial interest in Xsphera Biosciences, and is a consultant for Qiagen/N of One. D.A.B. has also received research funding from Novartis, Gilead Sciences, BMS, and Lilly Oncology.
Keywords
- cancer
- microfluidics
- microvasculatures
- perfusable
- physiological geometry
ASJC Scopus subject areas
- General Chemistry
- General Materials Science
