A Robust Method for Perfusable Microvascular Network Formation In Vitro

Zhengpeng Wan, Amy X. Zhong, Shun Zhang, Georgios Pavlou, Mark F. Coughlin, Sarah E. Shelton, Huu Tuan Nguyen, Jochen H. Lorch, David A. Barbie, Roger D. Kamm*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Micropost-based microfluidic devices are widely used for microvascular network (MVN) formation in diverse research fields. However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key characteristics of MVN formation is investigated and a robust two-step seeding strategy to generate perfusable physiological MVNs in microfluidic devices is established.

Original languageEnglish (US)
Article number2200143
JournalSmall Methods
Issue number6
StatePublished - Jun 30 2022


  • cancer
  • microfluidics
  • microvasculatures
  • perfusable
  • physiological geometry

ASJC Scopus subject areas

  • General Chemistry
  • General Materials Science


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