A role for heat shock factor 1 in hypercapnia-induced inhibition of inflammatory cytokine expression

Hypercapnia, elevated levels of CO₂ in the blood, is a known marker for poor clinical prognosis and is associated with increased mortality in patients hospitalized with both bacterial and viral pneumonias. Although studies have established a connection between elevated CO₂ levels and poor pneumonia outcomes, a mechanistic basis of this association has not yet been established. We previously reported that hypercapnia inhibits expression of key NF-kB–regulated, innate immune cytokines, TNF-a, and IL-6, in LPS-stimulated macrophages in vitro and in mice during Pseudomonas pneumonia. The transcription factor heat shock factor 1 (HSF1) is important in maintaining proteostasis during stress and has been shown to negatively regulate NF-kB activity. In this study, we tested the hypothesis that HSF1 activation in response to hypercapnia results in attenuated NF-kB–regulated gene expression. We found that hypercapnia induced the protein expression and nuclear accumulation of HSF1 in primary murine alveolar macrophages and in an alveolar macrophage cell line (MH-S). In MH-S cells treated with short interfering RNA targeting Hsf1, LPS-induced IL-6 and TNF-a release were elevated during exposure to hypercapnia. Pseudomonas-infected Hsf1^+/+ (wild-type) mice, maintained in a hypercapnic environment, showed lower levels of IL-6 and TNF-a in bronchoalveolar lavage fluid and IL-1b in lung tissue than did infected mice maintained in room air. In contrast, infected Hsf1^+/2 mice exposed to either hypercapnia or room air had similarly elevated levels of those cytokines. These results suggest that hypercapnia-mediated inhibition of NF-kB cytokine production is dependent on HSF1 expression and/or activation.—Lu, Z., Casalino-Matsuda, S. M., Nair, A., Buchbinder, A., Budinger, G. R. S., Sporn, P. H. S., Gates, K. L. A role for heat shock factor 1 in hypercapnia-induced inhibition of inflammatory cytokine expression.