A scheme for 3-dimensional morphological reconstruction and force inference in the early c. Elegans embryo

Muzhi Xu, Yicong Wu, Hari Shroff, Min Wu*, Madhav Mani

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

In this study, we present a scheme for the reconstruction of cellular morphology and the inference of mechanical forces in the early C. elegans embryo. We have developed and bench-marked a morphological reconstruction scheme that transforms flourescence-based in vivo images of membranes into a point cloud of smoothed surface patches, which facilitates an accurate estimation of membrane curvatures and the angles between membranes. Assuming an isotropic and homogeneous distribution of tensions along individual membranes, we infer a pattern of forces that are 7% deviated from force balance at edges, and 10% deviated from the Young-Laplace relation across membranes. We demonstrate the stability of our inference scheme via a sensitivity analysis, and the reproducibility of our image-analysis and force inference pipelines.

Original languageEnglish (US)
Article numbere0199151
JournalPloS one
Volume13
Issue number7
DOIs
StatePublished - Jul 2018

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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