@article{fd028645536442329ce981f76f747293,
title = "A scheme for 3-dimensional morphological reconstruction and force inference in the early c. Elegans embryo",
abstract = "In this study, we present a scheme for the reconstruction of cellular morphology and the inference of mechanical forces in the early C. elegans embryo. We have developed and bench-marked a morphological reconstruction scheme that transforms flourescence-based in vivo images of membranes into a point cloud of smoothed surface patches, which facilitates an accurate estimation of membrane curvatures and the angles between membranes. Assuming an isotropic and homogeneous distribution of tensions along individual membranes, we infer a pattern of forces that are 7% deviated from force balance at edges, and 10% deviated from the Young-Laplace relation across membranes. We demonstrate the stability of our inference scheme via a sensitivity analysis, and the reproducibility of our image-analysis and force inference pipelines.",
author = "Muzhi Xu and Yicong Wu and Hari Shroff and Min Wu and Madhav Mani",
note = "Funding Information: This work was supported by Engineering Science and Applied Mathematics at Northwestern University; Simons foundation Mathematical Modeling of Living Systems (https://www. simonsfoundation.org/mathematics-physical-sciences/simons-investigators/simons-investigators-awardees/); and Intramural Research Programs of the National Institute of Biomedical Imaging and Bioengineering. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.",
year = "2018",
month = jul,
doi = "10.1371/journal.pone.0199151",
language = "English (US)",
volume = "13",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "7",
}